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首页> 外文期刊>RSC Advances >Biomolecule incorporated poly-3-caprolactone nanofibrous scaffolds for enhanced human meniscal cell attachment and proliferation
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Biomolecule incorporated poly-3-caprolactone nanofibrous scaffolds for enhanced human meniscal cell attachment and proliferation

机译：生物分子掺入聚-3-己内酮纳米纤维支架，用于增强人半月板的附着和增殖

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摘要

The present study investigates the impact of biomolecule (biotin and galactose) incorporated poly-epsilon-caprolactone (PCL) nanofibrous scaffolds on attachment and proliferation of human meniscal cells by three modes of biomolecule supplementation. Two different ratios of biomolecules like biotin and galactose were incorporated in nanofibers using a standardized electrospinning process. Surface morphologies of control and biomolecule incorporated nanofibers were analyzed by field emission scanning electron microscope (FESEM). The presence of the biomolecules in the nanofibers was confirmed by Fourier Transform Infrared (FTIR) Spectroscopy. The biodegradability of pure PCL and biomolecules incorporated nanofibers was determined. The biomolecule embedded inside PCL nanofibrous scaffolds were studied in terms of DNA content and extra cellular matrix (ECM) (glycosaminoglycans (GAG) and collagen) for meniscus cell attachment, growth and proliferation with and without addition of these biomolecules (in the scaffold and in the medium). FESEM and fluorescence microscopic studies were used to confirm cell proliferation on the surface of the scaffolds. In vitro human meniscal cell culture study revealed that galactose incorporation was more efficient when compared to biotin. Enhanced meniscal cell attachment and proliferation were achieved when half of the biomolecule was inside the nanofiber and the other half was in the medium. This approach to improve the cell attachment onto the scaffold is a promising strategy for meniscal tissue engineering.
机译：本研究调查了生物分子（生物素和半乳糖）的影响掺入了多ε-己内酯（PCL）纳米纤维支架对人半月细胞的附着和增殖的三种生物分子补充。使用标准化的静电纺丝法加入了生物素和半乳糖等两种不同的生物分子比例。通过现场发射扫描电子显微镜（FESEM）分析了对照和生物分子的表面形态掺入纳米纤维。通过傅里叶变换红外（FTIR）光谱证实纳米纤维中的生物分子的存在。确定纯PCL和生物分子的生物降解性掺入纳米纤维。在DNA含量和额外的细胞基质（ECM）（糖胺聚糖（GAG）和胶原蛋白）方面研究了嵌入PCL纳米纤维支架中的生物分子，用于弯月面细胞附着，生长和增殖，并且在不添加这些生物分子（在支架中，媒介）。 FeSEM和荧光显微镜研究用于确认支架表面的细胞增殖。体外人半月板细胞培养研究表明，与生物素相比，半乳糖掺入更有效。当一半的生物分子在纳米纤维内部且另一半在培养基中时，实现了增强的半月板附着和增殖。这种改善电池附着在脚手架上的方法是半月板组织工程的有希望的策略。

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  • 来源
    《RSC Advances》 |2015年第90期|共10页
  • 作者

    Gopinathan J.; Mano Steffie; Elakkiya V.; Pillai Mamatha M.; Sahanand K. Santosh; Rai B. K. Dinakar; Selvakumar R.; Bhattacharyya Amitava;

  • 作者单位

    PSG Inst Adv Studies Adv Text &

    Polymer Res Lab Coimbatore 641004 Tamil Nadu India;

    PSG Inst Adv Studies Adv Text &

    Polymer Res Lab Coimbatore 641004 Tamil Nadu India;

    PSG Inst Adv Studies Tissue Engn Lab Coimbatore 641004 Tamil Nadu India;

    PSG Inst Adv Studies Tissue Engn Lab Coimbatore 641004 Tamil Nadu India;

    Ortho One Orthopaed Special Ctr Dept Orthopaed Surg Coimbatore 641005 Tamil Nadu India;

    PSG Inst Med Sci &

    Res Dept Orthopaed Coimbatore 641004 Tamil Nadu India;

    PSG Inst Adv Studies Tissue Engn Lab Coimbatore 641004 Tamil Nadu India;

    PSG Inst Adv Studies Adv Text &

    Polymer Res Lab Coimbatore 641004 Tamil Nadu India;

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  • 正文语种 eng
  • 中图分类 化学;
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