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首页> 外文期刊>RSC Advances >Electrochemically reduced graphene oxide supported poly(indole-5-carboxylic acid) nanocomposite for genosensing application
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Electrochemically reduced graphene oxide supported poly(indole-5-carboxylic acid) nanocomposite for genosensing application

机译：电化学还原的石墨烯氧化物负载的聚（吲哚-5-羧酸）纳米复合材料用于烧伤胶质胶版

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摘要

In this paper, the poly(indole-5-carboxylic acid) (PICA) was electropolymerized on a pre-obtained electrochemically reduced graphene oxide (ERGNO) membrane to develop a novel direct DNA electrochemical biosensor. Due to the unique properties of graphene, conjugated indole-5-carboxylic acid (ICA) monomers tended to be adsorbed on the graphene plane by pi-pi* stacking and the electropolymerization efficiency was greatly improved, resulting in an enhanced electrochemical response of PICA. The obtained conjugated PICA/ERGNO nanocomposite not only served as a substrate for DNA immobilization but also reflected the electrochemical transduction originating from DNA immobilization and hybridization without any complicated labeling or outer indicators. The immobilization of the probe ssDNA was successfully conducted via noncovalent assembly ascribed to the pi-pi* interaction between the conjugated nanocomposite and DNA bases. After the hybridization of the probe ssDNA with the target DNA, the formation of double-helix structure induced the resulted dsDNA to be released from the surface of the conjugated nanocomposite, accompanied with the self-signal regeneration of the nanocomposite ("signal-on"). The dynamic range of this DNA biosensor for detecting the complementary target of BCR/ABL fusion gene from chronic myelogenous leukemia was from 1.0 x 10(-16) to 1.0 x 10(-9) mol L-1 using electrochemical impedance spectroscopy, and the detection limit was 2.3 x 10(-17) mol L-1.
机译：在本文中，将聚（吲哚-5-羧酸）（PICA）在预先获得的电化学还原的石墨烯氧化物（ERGNO）膜上是电聚合以开发一种新型直接DNA电化学生物传感器。由于石墨烯的独特性质，通过PI-PI *堆叠将缀合的吲哚-5-羧酸（ICA）单体倾向于被PI-PI *堆叠吸附在石墨烯平面上，并且大大提高，导致了PICA的电化学响应增强了电化学响应。所得缀合的PICA / ERGNO纳米复合物不仅用作DNA固定化的基材，而且还反映了源自DNA固定化和杂交的电化学转导，而没有任何复杂的标记或外部指示剂。通过在缀合的纳米复合材料和DNA碱基之间的PI-PI *相互作用上成功地通过非共价组件成功地进行探针SSDNA的固定化。在探针SSDNA与靶DNA的杂交之后，将双螺旋结构的形成诱导从共轭纳米复合材料的表面释放所得的DSDNA，伴随着纳米复合材料的自信再生（“信号-OP” ）。该DNA生物传感器的动态范围用于检测来自慢性髓性白血病的BCR / ABL融合基因的互补靶标，使用电化学阻抗光谱法为1.0×10（ - 16）至1.0×10（-9）摩尔L-1，以及检出限为2.3×10（-17）摩尔L-1。

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  • 来源
    《RSC Advances》 |2015年第125期|共7页
  • 作者

    Zhang Wei;

  • 作者单位

    Linyi Univ Sch Chem &

    Chem Engn Linyi 276005 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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