• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Gellan gum-coated gold nanorods: an intracellular nanosystem for bone tissue engineering
【24h】

Gellan gum-coated gold nanorods: an intracellular nanosystem for bone tissue engineering

机译：Gellan Gum涂层金纳米棒：骨组织工程的细胞内纳米系统

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gold nanorods (AuNRs) have emerged as an exceptional nanotool for a myriad of applications ranging from cancer therapy to tissue engineering. However, their surface modification with biocompatible and stabilizing biomaterials is crucial to allow their use in a biological environment. Herein, low-acyl gellan gum (GG) was used to coat AuNRs surface, taking advantage of its stabilizing, biocompatible and gelling features. The layer-by-layer based strategy implied the successive deposition of poly(acrylic acid), poly(allylamine hydrochloride) and GG, which allowed the formation of a GG hydrogel-like shell with 7 nm thickness around individual AuNRs. Stability studies in a wide range of pH and salt concentrations showed that the polysaccharide coating can prevent AuNRs aggregation. Moreover, a reversible pH-responsive feature of the nanoparticles was observed. Cytocompatibility and osteogenic ability of GG-coated AuNRs were also addressed. After 14 days of culturing within SaOS-2, an osteoblast-like cell line, in vitro studies revealed that AuNRs-GG exhibit no cytotoxicity, were internalized by the cells and localized inside lysosomes. AuNRs-GG combined with osteogenic media enhanced by two fold the mineralization capacity, as compared to cells exposed to osteogenic media alone. The proposed system has shown interesting features for osteogenesis, and further insights might be relevant for drug delivery, tissue engineering and regenerative medicine.
机译：金纳米棒（AUNRS）已成为一种卓越的纳米工具，用于癌症治疗与组织工程的癌症。然而，它们具有生物相容性和稳定的生物材料的表面改性至关重要，以便在生物环境中使用。在此，使用低酰基Gellan Gum（GG）来涂覆AUNRS表面，利用其稳定，生物相容性和胶凝特征。基于层的层基的策略暗示了聚（丙烯酸），聚（盐酸丙烯酸）和GG的连续沉积，其允许形成具有7nm厚度的GG水凝胶状壳，在各个AUNR周围。各种pH和盐浓度的稳定性研究表明，多糖涂层可以预防AUNRS聚集。此外，观察到纳米颗粒的可逆性pH-响应特征。还解决了GG涂层AUNR的细胞偶联和成骨能力。在Saos-2中培养14天后，体外研究培养的型成骨细胞样细胞系显示，AUNRS-GG没有表现出细胞毒性，被细胞内化并局部内部化溶酶体内化。与单独暴露于成骨介质的细胞相比，AUNRS-Gg与成骨培养基增强了两倍的矿化容量。该拟议的系统表明了骨质发生的有趣特征，进一步的见解可能与药物递送，组织工程和再生医学相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号