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首页> 外文期刊>RSC Advances >A label-free electrochemical immunosensor with a novel signal production and amplification strategy based on three-dimensional pine-like Au-Cu nanodendrites
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A label-free electrochemical immunosensor with a novel signal production and amplification strategy based on three-dimensional pine-like Au-Cu nanodendrites

机译：一种无标记的电化学免疫传感器，具有基于三维松树样Au-Cu纳秒的新型信号产生和扩增策略

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摘要

A simple and novel label-free electrochemical immunosensor based on a bimetallic alloy was fabricated in this work. Three-dimensional (3D) pine-like Au-Cu nanodendrites (NDs) electrodeposited on the bare glassy carbon electrode (GCE) were employed as transducing materials to achieve the production and amplification of the electrochemical signal. The electrochemical detection was based on the fact that the immunocomplex can hinder the direct electron transfer in the redox process of Cu. Cu, instead of the traditional redox electron mediators, was exploited as a new signal producer. Au with good electron transfer ability could act as signal amplifier and capture antibodies. In addition, the 3D pine-like morphology of Au-Cu NDs with large specific surface area was beneficial to the immobilization of antibodies and the electron transfer between protein and metal. Under the optimal conditions, by using carbohydrate antigen 724 (CA724) as the target analyte, the label-free electrochemical immunosensor exhibited a wide linear range from 0.5 pg mL(-1) to 500 ng mL(-1) and a low detection limit of 0.25 pg mL(-1). The proposed immunosensor also displayed a satisfying electrochemical performance with good reproducibility, selectivity and stability. In this sense, this novel method will provide a new promising application for the clinical immunoassay of other biomarkers.
机译：在这项工作中制造了一种基于双金属合金的简单和新的无标签的电化学免疫传感器。在裸玻璃碳电极（GCE）上电沉积在裸玻璃碳电极（GCE）上的三维（3D）松树样AU-Cu纳秒（NDS）作为转换材料，以实现电化学信号的生产和扩增。电化学检测基于免疫激散能阻碍Cu的氧化还原过程中的直接电子转移的事实。 CU，代替传统的氧化还原电子介质，被利用为新的信号生产商。具有良好电子转移能力的Au可以充当信号放大器和捕获抗体。此外，具有大特异性表面积的Au-Cu Nds的3D松树形态有利于固定抗体和蛋白质和金属之间的电子转移。在最佳条件下，通过使用碳水化合物抗原724（CA724）作为靶分析物，无标记的电化学免疫传感器在0.5pg ml（-1）至500ng ml（-1）和低检测极限上表现出宽的线性范围0.25pg ml（-1）。所提出的免疫传感器还显示出具有良好的再现性，选择性和稳定性的令人满意的电化学性能。从这个意义上讲，这种新方法将为其他生物标志物的临床免疫分析提供新的有希望的申请。

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  • 来源
    《RSC Advances》 |2015年第40期|共8页
  • 作者

    Wang Yulan; Li Yueyun; Ma Hongmin; Ren Xiang; Cao Wei; Yan Tao; Wei Qin;

  • 作者单位

    Univ Jinan Shandong Univ Sch Chem &

    Chem Engn Key Lab Chem Sensing &

    Anal Jinan 250022 Peoples R China;

    Shandong Univ Technol Sch Chem Engn Zibo 255049 Peoples R China;

    Univ Jinan Shandong Univ Sch Chem &

    Chem Engn Key Lab Chem Sensing &

    Anal Jinan 250022 Peoples R China;

    Univ Jinan Shandong Univ Sch Chem &

    Chem Engn Key Lab Chem Sensing &

    Anal Jinan 250022 Peoples R China;

    Univ Jinan Shandong Univ Sch Chem &

    Chem Engn Key Lab Chem Sensing &

    Anal Jinan 250022 Peoples R China;

    Univ Jinan Shandong Univ Sch Chem &

    Chem Engn Key Lab Chem Sensing &

    Anal Jinan 250022 Peoples R China;

    Univ Jinan Shandong Univ Sch Chem &

    Chem Engn Key Lab Chem Sensing &

    Anal Jinan 250022 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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