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首页> 外文期刊>RSC Advances >Bio-augmentative volatile fatty acid production from waste activated sludge hydrolyzed at pH 12
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Bio-augmentative volatile fatty acid production from waste activated sludge hydrolyzed at pH 12

机译：生物增强的挥发性脂肪酸从pH1 12的废物活性污泥中产生

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摘要

Although many pretreatment methods are employed to enhance the hydrolysis of waste activated sludge (WAS), the bioconversion of soluble complex substrates needs improvement to produce higher volatile fatty acids (VFAs). This study isolated a new strain of Citrobacter freundii, which was first used as a VFAs-producing strain under alkaline pH. A bio-augmentative test achieved the highest total production of VFAs (2425.2 mg COD per L) and offered a preferred fermentation method, which yielded more acetic acid (65.0%) than a sludge-only test (50.1%). Moreover, the bio-augmentative test could produce evident synergistic effects on the utilization of soluble complex substrates between WAS and the Citrobacter freundii strain. Furthermore, the addition of carbohydrates takes precedence over the adjustment of pH in the production of VFAs, which suggested that the regulation of the ratio of protein to carbohydrates (PN/PS) was more practical and more convenient. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) results revealed that the Citrobacter freundii strain was the dominant bacterial community in the bio-augmentative test and some indigenous species were affected due to competition for nutrients with Citrobacter freundii.
机译：尽管使用许多预处理方法来增强废物活性污泥的水解（是），但可溶性复合物底物的生物转化需要改善以产生更高的挥发性脂肪酸（VFA）。本研究分离出一种新的植物杆菌菌株，首先用作在碱性pH下产生的VFA产生菌株。生物增强测试达到了VFA的总产量（每1425.2mg COD鳕鱼），并提供了优选的发酵方法，其含有比仅污泥测试（50.1％）的乙酸（65.0％）。此外，生物增强试验可以对含有溶质复合底物的利用产生明显的协同作用和柠檬杆菌Freundii菌株。此外，添加碳水化合物优先于对VFA的生产调整pH的调整，这表明蛋白质与碳水化合物（PN / PS）的比例更加实用，更方便。聚合酶链反应变性梯度凝胶电泳（PCR-DGGE）结果表明，Citrobacter Freundii菌株是生物增强试验中的显性细菌群落，并且由于培养氟碳菌培养物的竞争，一些土着物种受到影响。

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  • 来源
    《RSC Advances》 |2015年第62期|共7页
  • 作者

    Yang Xue; Wen Lei; Liu Xiang; Chen Si; Wang Yayi; Wan Chunli;

  • 作者单位

    Tongji Univ Postdoctoral Res Stn Civil Engn Shanghai 200092 Peoples R China;

    Fudan Univ Dept Environm Sci &

    Engn Shanghai 200433 Peoples R China;

    Fudan Univ Dept Environm Sci &

    Engn Shanghai 200433 Peoples R China;

    Fudan Univ Dept Environm Sci &

    Engn Shanghai 200433 Peoples R China;

    Tongji Univ Coll Environm Sci &

    Engn State Key Lab Pollut Control &

    Resources Reuse Shanghai 200092 Peoples R China;

    Fudan Univ Dept Environm Sci &

    Engn Shanghai 200433 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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