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Arginyl-glutamine dipeptide attenuates experimental colitis by enhancing antioxidant function and inhibiting nuclear factor-kappaB

机译：通过增强抗氧化功能和抑制核因子 - κB，氨基 - 谷氨酰胺二肽衰减实验性结肠炎

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摘要

This study aimed to investigate the effect and underlying mechanism of Arginyl-glutamine (AG) dipeptide on dextran sulfate sodium (DSS)-induced colitis by in vivo and in vitro models. Acute colitis was induced in ICR mice by administering 5% DSS. 1% AG and 0.5% glutamine and 0.5% arginine were fed via mixing in the basic diet after colitis induction. The results showed that AG could reverse the diverse effects on average daily weight gain, colon weight, rectal bleeding score, diarrhea score, and histological score caused by DSS. Meanwhile, dietary AG supplementation attenuated DSS-induced oxidative stress and improved the antioxidant system in mice. Although AG failed to improve intestinal dysfunction, inflammatory response was markedly reduced in DSS-induced colitis after AG treatment. Ultimately, AG inhibited IKK phosphorylation and subsequent nuclear factor-kappaB (NF-kappa B) activity and its translocation into the nucleus. From the in vitro study, AG was found to improve cell viability, antioxidant function, and monolayer permeability and reduce inflammatory response in DSS-challenged Caco-2 cells. In conclusion, AG protected mice against DSS-induced colitis via increasing the antioxidant system and down-regulating proinflammatory cytokines. The inhibition of NF-kappa B activation might be part of the mechanism underlying the effects of AG on the colitis model.
机译：本研究旨在通过体内和体外模型来研究精氨酰谷氨酰胺（AG）的二肽对葡聚糖硫酸钠（DSS）诱发的结肠炎的影响和潜在机制。急性结肠炎通过给予5％DSS ICR小鼠诱导。 1％Ag和0.5％的谷氨酰胺和0.5％的精氨酸分别经由结肠炎诱导后混合在基础饲料喂养。结果表明，AG能逆转平均日增重，结肠重量，直肠出血分数，腹泻得分，并造成DSS组织学分数不同的影响。同时，膳食补充AG衰减DSS诱导的氧化应激和改善的在小鼠中的抗氧化剂体系。虽然AG未能改善肠功能障碍，炎症反应被显着在AG处理后DSS诱导的结肠炎降低。最终，AG抑制IKK磷酸化和随后的核因子-κB（NF-κB的）活性和其易位到细胞核中。从体外研究，AG发现提高细胞活力，抗氧化功能，和单层渗透性和减少在DSS激发的Caco-2细胞的炎症反应。总之，通过公司增加抗氧化系统和下调促炎细胞因子免受DSS诱导的结肠炎的小鼠。 NF-κB的活化的抑制可能是潜在的AG对结肠炎模型中的作用的机构的一部分。

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  • 来源
    《RSC Advances》 |2015年第112期|共9页
  • 作者

    Yu Hua; Dong Mingjun; Xu Yidong; He Ning; Dai Xiaoyu; Li Keqiang;

  • 作者单位

    Ningbo 2 Hosp Dept Anal Ningbo 315010 Zhejiang Peoples R China;

    Ningbo 2 Hosp Dept Anal Ningbo 315010 Zhejiang Peoples R China;

    Ningbo 2 Hosp Dept Anal Ningbo 315010 Zhejiang Peoples R China;

    Ningbo 2 Hosp Dept Anal Ningbo 315010 Zhejiang Peoples R China;

    Ningbo 2 Hosp Dept Anal Ningbo 315010 Zhejiang Peoples R China;

    Ningbo 2 Hosp Dept Anal Ningbo 315010 Zhejiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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