2 '-N-Guanidino, 4 '-C-ethylene bridged thymidine (GENA-T) modified oligonucleotide exhibits triplex formation with excellent enzymatic stability

Barman Jharna; Gurav Deepanjali; Oommen Oommen P.; Varghese Oommen P.

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2 '-N-Guanidino, 4 '-C-ethylene bridged thymidine (GENA-T) modified oligonucleotide exhibits triplex formation with excellent enzymatic stability

机译：2'-n-guanidino，4'-C-乙烯桥接胸苷（Gena-T）改性寡核苷酸表现出具有优异酶促稳定性的三链形成

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Here we present the synthesis and characterization of a new 2'-N-Guanidino, 4'-C-ethylene bridged thymidine (GENA-T) modified oligonucleotide possessing North-locked sugar conformation. Incorporation of GENA-T nucleotide though did not change the thermal stability of the oligonucleotides toward the complementary RNA; it significantly increased the stability of the parallel triplex at pH 7. The melting temperature of the triplex was increased by +9.5 degrees C as compared to that of the isosequential unmodified sequence. Moreover this modification imparted exceptional nuclease stability to the oligonucleotides for over 33 h. This study clearly demonstrates that GENA-T modified oligonucleotides could improve triplex formation with phenomenal enzymatic stability and could be used for various biomedical applications.

机译：在这里，我们介绍了新的2'-n-guaNidino，4'-c-乙烯桥接胸苷（Gena-T）改性寡核苷酸的合成和表征，所述寡核苷酸具有北锁糖构象。掺入Gena-T核苷酸虽然不会改变寡核苷酸朝向互补RNA的热稳定性; 它显着提高了pH7的平行三链接的稳定性。与异质未改性序列相比，三醇的熔化温度增加+ 9.5℃。此外，该改性赋予寡核苷酸超过33小时的特殊核酸酶稳定性。该研究清楚地证明了Gena-T改性的寡核苷酸可以改善具有现象酶促稳定性的三醇地层，可用于各种生物医学应用。

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《RSC Advances》 |2015年第16期|共4页
作者
Barman Jharna; Gurav Deepanjali; Oommen Oommen P.; Varghese Oommen P.;
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Indian Stat Inst Div Biol Sci Agr &

Ecol Res Unit Kolkata 700108 India;

Uppsala Univ Dept Chem Polymer Chem Div Angstrom Lab S-75121 Uppsala Sweden;

Uppsala Univ Dept Chem Polymer Chem Div Angstrom Lab S-75121 Uppsala Sweden;

Uppsala Univ Dept Chem Polymer Chem Div Angstrom Lab S-75121 Uppsala Sweden;

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正文语种 eng
中图分类化学;
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1. 2 '-N-Guanidino, 4 '-C-ethylene bridged thymidine (GENA-T) modified oligonucleotide exhibits triplex formation with excellent enzymatic stability [J] . Barman Jharna, Gurav Deepanjali, Oommen Oommen P., RSC Advances . 2015,第16期

机译：2'-n-guanidino，4'-C-乙烯桥接胸苷（Gena-T）改性寡核苷酸表现出具有优异酶促稳定性的三链形成
2. Conformationally constrained 2 '-N,4 '-C-ethylene-bridged thymidine (aza-ENA-T): Synthesis, structure, physical, and biochemical studies of aza-ENA-T-modified oligonucleotides [J] . Varghese OP, Barman J, Pathmasiri W, Journal of the American Chemical Society . 2006,第47期

机译：构象受限的2'-N，4'-C-乙烯桥胸腺嘧啶核苷（氮杂-ENA-T）：氮杂-ENA-T修饰的寡核苷酸的合成，结构，物理和生化研究
3. 2′-O,4′-C-ethylene bridged nucleic acid modification enhances pyrimidine motif triplex-forming ability under physiological condition [J] . TorigoeH., SatoN., NagasawaN. The Journal of Biochemistry . 2012,第1期

机译：2'-O，4'-C-乙烯桥连的核酸修饰在生理条件下增强嘧啶基序三链体形成能力
4. Reversible photo-regulation of DNA duplex and triplex formation by using modified oligonucleotides bearing azobenzene [C] . Xingguo Liang, Takayuki Yoshida, Akira Yamazawa, Pacific polymer conference preprints . 1999

机译：通过使用含偶氮核苷酸的改性寡核苷酸可逆光调节DNA双链和三链形成
5. DNA triplex formation by base, backbone, and sugar-ring modified oligonucleotides with HIV targets. [D] . Cassidy, Rachel Ann. 2004

机译：通过具有HIV靶标的碱基，主链和糖环修饰的寡核苷酸形成DNA三链体。
6. Single strand targeted triplex-formation. Destabilization of guanine quadruplex structures by foldback triplex-forming oligonucleotides. [O] . E R Kandimalla, S Agrawal 1995

机译：单链靶向的三链体形成。鸟嘌呤四联体结构的失稳通过形成折返三链体的寡核苷酸来实现。
7. 2P-124 Promotion of Pyrimidine Motif Triplex Formation at Physiological PH by 2'-O, 4'-C-Ethylene Bridged Nucleic Acid (ENA) Modification(The 46th Annual Meeting of the Biophysical Society of Japan) [O] . Nobuyuki Nagasawa, Hidetaka Torigoe 2008

机译：2P-124在生理pH下促进嘧啶基序三氯联合物形成2'-O，4'-C-乙烯桥接核酸（ENA）修改（日本生物物理学学会第46次年会）
8. Preparation of New Perfluoroether Fluids Exhibiting Excellent Thermal-Oxidative Stabilities [R] . Jones, W. R., Bierschenk, T. R., Juhlke, T. J., 1986

机译：新型全氟醚流体的制备具有良好的热氧化稳定性

2 '-N-Guanidino, 4 '-C-ethylene bridged thymidine (GENA-T) modified oligonucleotide exhibits triplex formation with excellent enzymatic stability

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