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首页> 外文期刊>Acta biomaterialia >Synergistic effect of surface modification and scaffold design of bioplotted 3-D poly-ε-caprolactone scaffolds in osteogenic tissue engineering.
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Synergistic effect of surface modification and scaffold design of bioplotted 3-D poly-ε-caprolactone scaffolds in osteogenic tissue engineering.

机译：生物成图的3-D聚-ε-己内酯支架的表面改性和支架设计在成骨组织工程中的协同作用。

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摘要

The hydrophobic nature and the regular scaffold architecture of bioplotted poly(ε-caprolactone) (PCL) scaffolds present some hurdles for homogeneous tissue formation and differentiation. The current hypothesis is that a synergistic effect of applied surface modification and scaffold design enhances colonization and osteogenic differentiation. First, PCL scaffolds with a 0/90° lay-down pattern (0/90) were plotted and subjected to an oxygen plasma (O2) or multistep surface modification, including post-argon 2-amino-ethylmethacrylate grafting (AEMA), followed by immobilization of gelatin type B (gelB) and physisorption of fibronectin (gelB Fn). Secondly, scaffolds of different designs were plotted (0/90° shift (0/90 S), 0/45° and 0/90° with narrow pores (0/90 NP)) and subjected to the double protein coating. Preosteoblasts were cultured on the scaffolds and the seeding efficiency, colonization and differentiation were studied. The data revealed that a biomimetic surface modification improved colonization (gelB Fn>gelB>AEMA>O2). Compact scaffold architectures (0/90 NP, 0/45, 0/90 S>0/90) positively influenced the seeding efficiency and differentiation. Interestingly, the applied surface modification had a greater impact on colonization than the scaffold design. In conclusion, the combination of a double protein coating with a compact design enhances tissue formation in the plotted PCL scaffolds.
机译：生物印迹聚（ε-己内酯）（PCL）支架的疏水性和常规支架结构为均一的组织形成和分化提供了一些障碍。当前的假设是，应用表面修饰和支架设计的协同效应会增强定植和成骨分化。首先，绘制具有0/90°放置图案（0/90）的PCL支架，并进行氧等离子体（O2）或多步表面修饰，包括后氩2-氨基乙基甲基丙烯酸甲酯接枝（AEMA），通过固定B型明胶（gelB）和纤连蛋白（gelB Fn）的物理吸附。其次，绘制不同设计的支架（0/90°位移（0/90 S），0/45°和0/90°且具有窄孔（0/90 NP）），并进行双蛋白包被。将前成骨细胞培养在支架上，研究播种效率，定植和分化。数据显示仿生表面修饰改善了定植（gelB Fn> gelB> AEMA> O2）。紧凑型支架架构（0/90 NP，0 / 45、0 / 90 S> 0/90）积极影响播种效率和分化。有趣的是，所施加的表面修饰比支架设计对定植的影响更大。总之，双层蛋白涂层与紧凑型设计的结合可增强绘制的PCL支架中的组织形成。

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