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首页> 外文期刊>Acta biomaterialia >Amphiphilic peptide-loaded nanofibrous calcium phosphate microspheres promote hemostasis in vivo
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Amphiphilic peptide-loaded nanofibrous calcium phosphate microspheres promote hemostasis in vivo

机译：两亲性肽负载的纳米纤维磷酸钙微球促进体内止血

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摘要

Most fatalities from trauma occur due to severe blood loss. There is a need for improved hemostatic biomaterials that can address this problem. The aim of this study was to determine the in vivo efficacy of nanofibrous microspheres (NFM) loaded with hemostatic peptides, specifically ideal amphipathic peptides (IAP) that have been demonstrated to possess both procoagulant and antifibrinolyic activities. We demonstrate that IAP can be coupled to NFM (IAP-NFM) to form matrices that exhibit substantial hemostatic activity. IAP-NFM matrices were compared to a commercial zeolitic hemostatic biomaterial (QuikClot) and have superior efficacy in reducing bleeding in vivo. In both a murine tail transection and a murine hepatic injury model, bleeding times were significantly reduced (P < 0.05) with the use of IAP-NFM as compared with equal masses of either QuikClot or NFM alone, or no treatment. Importantly, histological examination revealed no tissue injury when IAP-NFM or NFM were applied to hepatic lacerations. In contrast, QuikClot caused widespread hepatocyte degeneration and necrosis, with higher average injury zone thickness as determined by semiquantitative analysis. In summary, NFM was able to maintain the pro-coagulant properties of IAP in our preclinical model, caused no observable tissue damage at the site of application and had better performance than QuikClot controls.
机译：创伤造成的大多数死亡是由于严重失血造成的。需要改善的止血生物材料以解决该问题。这项研究的目的是确定载有止血肽，特别是理想的两亲性肽（IAP）的纳米纤维微球（NFM）的体内功效，已证明其具有促凝血和抗纤溶活性。我们证明，IAP可以耦合到NFM（IAP-NFM）形成具有实质性止血活性的基质。将IAP-NFM基质与商用沸石止血生物材料（QuikClot）进行了比较，在降低体内出血方面具有出色的功效。在小鼠尾部横断和小鼠肝损伤模型中，与同等质量的QuikClot或NFM或不进行治疗相比，使用IAP-NFM可以显着减少出血时间（P <0.05）。重要的是，当将IAP-NFM或NFM应用于肝撕裂伤时，组织学检查没有发现组织损伤。相反，QuikClot引起广泛的肝细胞变性和坏死，如半定量分析所确定的，平均损伤区厚度更高。总之，NFM能够在我们的临床前模型中保持IAP的促凝特性，在应用部位未引起可观察到的组织损伤，并且比QuikClot对照具有更好的性能。

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