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首页> 外文期刊>Acta biomaterialia >Immobilization of sodium alginate sulfates on polysulfone ultrafiltration membranes for selective adsorption of low-density lipoprotein
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Immobilization of sodium alginate sulfates on polysulfone ultrafiltration membranes for selective adsorption of low-density lipoprotein

机译：海藻酸钠硫酸钠固定在聚砜超滤膜上以选择性吸附低密度脂蛋白

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摘要

A novel method for the immobilization of sodium alginate sulfates (SAS) on polysulfone (PSu) ultrafiltration membranes to achieve selective adsorption of low-density lipoprotein (LDL) was developed, which involved the photoinduced graft polymerization of acrylamide on the membrane and the Hofmann rearrangement reaction of grafted acrylamide followed by chemical binding of SAS with glutaraldehyde. The surface modification processes were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy characterization. Zeta potential and water contact angle measurements were performed to investigate the surface charge and wettability of the membranes. An enzyme-linked immunosorbent assay was used to measure the binding of LDL on plain and modified PSu membranes. It was found that the PSu membrane immobilized with sodium alginate sulfates (PSu-SAS) greatly enhanced the selective adsorption of LDL from protein solutions and the absorbed LDL could be easily eluted with sodium chloride solution, indicating a specific and reversible binding of LDL to SAS, mainly driven by electrostatic forces. Furthermore, the PSu-SAS membrane showed good blood compatibility as examined by platelet adhesion. The results suggest that the PSu-SAS membranes are promising for application in simultaneous hemodialysis and LDL apheresis therapy.
机译：开发了一种将藻酸钠硫酸钠固定在聚砜（PSu）超滤膜上以实现低密度脂蛋白（LDL）选择性吸附的新方法，该方法涉及丙烯酰胺在膜上的光诱导接枝聚合和霍夫曼重排接枝丙烯酰胺反应，然后将SAS与戊二醛化学键合。表面改性过程通过衰减全反射傅里叶变换红外光谱和X射线光电子能谱表征得到证实。进行ζ电势和水接触角测量以研究膜的表面电荷和润湿性。酶联免疫吸附测定法用于测量LDL在平膜和改性PSu膜上的结合。研究发现，固定有藻酸钠硫酸钠（PSu-SAS）的PSu膜大大增强了LDL从蛋白质溶液中的选择性吸附，吸收的LDL可以很容易地用氯化钠溶液洗脱，表明LDL与SAS的特异性和可逆结合，主要由静电力驱动。此外，通过血小板粘附检查，PSu-SAS膜显示出良好的血液相容性。结果表明，PSu-SAS膜有望用于同时进行的血液透析和LDL血液分离术。

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