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首页> 外文期刊>Cytogenetic and genome research >The ZW micro-sex chromosomes of the Chinese soft-shelled turtle (Pelodiscus sinensis, Trionychidae, Testudines) have the same origin as chicken chromosome 15.
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The ZW micro-sex chromosomes of the Chinese soft-shelled turtle (Pelodiscus sinensis, Trionychidae, Testudines) have the same origin as chicken chromosome 15.

机译:中国soft的ZW微性染色体与鸡15号染色体具有相同的起源。中华turtle(Pelodiscus sinensis ,Trionychidae,Testudines)具有相同的起源。

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摘要

The Chinese soft-shelled turtle (Pelodiscus sinensis, Trionychidae, Testudines) has ZZ/ZW-type micro-sex chromosomes where the 18S-28S ribosomal RNA genes (18S-28S rDNA) are located. The W chromosome is morphologically differentiated from the Z chromosome by partial deletion and amplification of 18S-28S rDNA and W-specific repetitive sequences. We recently found a functional gene (TOP3B) mapped on the P. sinensis Z chromosome, which is located on chicken (Gallus gallus, GGA) chromosome 15. Then we cloned turtle homologues of 4 other GGA15-linked genes (GIT2, NF2, SBNO1, SF3A1) and localized them to P. sinensis chromosomes. The 4 genes all mapped on the Z chromosome, and 2 of them (SBNO1, SF3A1) were also localized to the W chromosome. Our mapping data suggest that at least one large inversion occurred between GGA15 and the P. sinensis Z chromosome, and that there are homologous regions in the distal portions of both the short and long arms between the Z and W chromosomes. W chromosomal differentiation in P. sinensis probably proceeded by the deletion of the proximal chromosomal region followed by 18S-28S rDNA amplification, after a paracentric inversion occurred at the breakpoints between the distal region of 18S-28S rDNA and the proximal region of SBNO1 on the Z chromosome.
机译:中国甲鱼(中华elo,Trionychidae,Testudines)具有ZZ / ZW型微性别染色体,位于18S-28S核糖体RNA基因(18S-28S rDNA)所在。通过部分缺失和扩增18S-28S rDNA和W特异性重复序列,W染色体在形态上与Z染色体有所区别。我们最近发现了在 P上定位的功能性基因( TOP3B )。位于鸡( Gallus gallus ,GGA)15号染色体上的sinensis Z染色体,然后克隆了其他4个与GGA15相关的基因( GIT2 , NF2 , SBNO1 , SF3A1 )并将其本地化为 P。中华染色体。全部位于Z染色体上的4个基因,其中2个( SBNO1 , SF3A1 )也位于W染色体上。我们的映射数据表明,GGA15和 P之间至少发生了一次大反转。中华绒螯蟹的Z染色体,并且Z和W染色体之间的长臂和短臂的远端都有一个同源区域。 P的W染色体分化。在18S-28S rDNA远端区域和 SBNO1 < / i>在Z染色体上。

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