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首页> 外文期刊>Acta crystallographica, Section D. Biological crystallography >Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals
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Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals

机译：插入染料可增强蛋白质晶体二次谐波成像中的对比度

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摘要

The second-harmonic generation (SHG) activity of protein crystals was found to be enhanced by up to similar to 1000-fold by the intercalation of SHG phores within the crystal lattice. Unlike the intercalation of fluorophores, the SHG phores produced no significant background SHG from solvated dye or from dye intercalated into amorphous aggregates. The polarization-dependent SHG is consistent with the chromophores adopting the symmetry of the crystal lattice. In addition, the degree of enhancement for different symmetries of dyes is consistent with theoretical predictions based on the molecular nonlinear optical response. Kinetics studies indicate that intercalation arises over a timeframe of several minutes in lysozyme, with detectable enhancements within seconds. These results provide a potential means to increase the overall diversity of protein crystals and crystal sizes amenable to characterization by SHG microscopy.
机译：发现通过晶体晶格中SHG phors的插入，蛋白质晶体的二次谐波生成（SHG）活性提高了近1000倍。与荧光团的嵌入不同，SHG团不会从溶剂化染料或嵌入无定形聚集体的染料中产生明显的背景SHG。偏振相关的SHG与采用晶格对称性的发色团一致。另外，染料不同对称性的增强程度与基于分子非线性光学响应的​​理论预测一致。动力学研究表明，在溶菌酶中，插入发生在几分钟的时间内，几秒钟内可检测到增强。这些结果提供了一种潜在的手段，可以增加蛋白质晶体的总体多样性和适合SHG显微镜表征的晶体大小。

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