...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics >Structural characterization of 1-deoxy-D-xylulose 5-phosphate Reductoisomerase from Vibrio vulnificus
【24h】

Structural characterization of 1-deoxy-D-xylulose 5-phosphate Reductoisomerase from Vibrio vulnificus

机译：vibrioulnificus的1-脱氧-d-木糖5-磷酸钙化酶酶的结构表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vibrio vulnificus, a gram-negative bacterium, is the leading cause of seafood-borne illnesses and mortality in the United States. Previous studies have identified metabolites 2-C-methylerythritol 4-phosphate (MEP) as being essential for V. vulnificus growth and function. It was shown that 1-deoxy-D-xylulose-5-phosphate reductoisomerase (Dxr) is a critical enzyme in the viability of V. vulnificus, and many other bacteria, as it catalyzes the rearrangement of 1-deoxy-D-xylulose-5-phosphate (Dxp) to 2-C-methylerythritol 4-phosphate (MEP) within the MEP pathway, found in plants and bacteria. The MEP pathway produces the isoprenoids, isopentenyl diphosphate and dimethylallyl pyrophosphate. In this study, we produced and structurally characterized V. vulnificus Dxr. The enzyme forms a dimeric assembly and contains a metal ion in the active site. Protein produced in Escherichia coli co-purifies with Mg2+ ions, however the Mg2+ cations may be substituted with Mn2+, as both of these metals may be utilized by Dxrs. These findings will provide a basis for the design of Dxr inhibitors that may find application as antimicrobial compounds.
机译：革命性的细菌是革命性的，是美国海鲜疾病和死亡率的主要原因。以前的研究已经将代谢物2-C-甲基纤维素4-磷酸酯（MEP）鉴定为V.Vulnificus生长和功能至关重要。结果表明，1-脱氧-D-木糖糖-5-磷酸氧化酯酶（DXR）是V.Vulnfificus和许多其他细菌的活力中的关键酶，因为它催化了1-脱氧-D-木糖苷的重排 - 在植物和细菌中发现的MEP途径内的5-磷酸盐（DXP）至2-C-甲基吡酰胺4-磷酸盐（MEP）。 MEP途径产生异戊二烯，异戊烯基二磷酸二磷酸和二甲基丙烯酸磷酸酯。在这项研究中，我们生产和结构表征了V.Vulnificus DXR。酶形成二聚体组件并在活性位点中含有金属离子。在大肠杆菌中产生的蛋白质与Mg 2 +离子共同净化，但是Mg 2 +阳离子可以被Mn2 +取代，因为这些金属两者可以通过DXRS使用。这些发现将为DXR抑制剂的设计提供依据，该抑制剂可以含有抗微生物化合物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号