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首页> 外文期刊>Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics >MS imaging and mass spectrometric synaptosome profiling identify PEP-19/pcp4 as a synaptic molecule involved in spatial learning in mice
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MS imaging and mass spectrometric synaptosome profiling identify PEP-19/pcp4 as a synaptic molecule involved in spatial learning in mice

机译：MS成像和质谱突触骨质体分析识别PEP-19 / PCP4作为涉及小鼠空间学习的突触分子

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The Morris water maze (MWM) spatial learning task has been demonstrated to involve a cognitive switch of action control to serve the transition from an early towards a late learning phase. However, the molecular mechanisms governing this switch are largely unknown.We employed MALDI MS imaging (MSI) to screen for changes in expression of small proteins in brain structures implicated in the different learning phases. We compared mice trained for 3 days and 30 days in the MWM, reflecting an early and a late learning phase in relation to the acquisition of a spatial learning task. An ion with m/z of 6724, identified as PEP-19/pcp4 by top-down tandem MS, was detected at higher intensity in the dorsal striatum of the late learning phase group compared with the early learning phase group. In addition, mass spectrometric analysis of synaptosomes confirmed the presence of PEP-19/pcp4 at the synapse. PEP-19/pcp4 has previously been identified as a critical determinant of synaptic plasticity in locomotor learning. O r findings extend PEP-19/pcp4 function to spatial learning in the forebrain and put MSI forward as a valid and unbiased research strategy for the discovery and identification of the molecular machinery involved in learning, memory and synaptic plasticity. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann
机译：已经证明了Morris水迷宫（MWM）空间学习任务涉及动作控制的认知开关，以从早期朝向晚期学习阶段服务过渡。然而，治疗该开关的分子机制很大程度上是未知的.WE使用MALDI MS成像（MSI）对不同学习阶段的脑结构中小蛋白表达的变化筛选。我们将培训3天和30天的小鼠与MWM进行了3天，反映了与收购空间学习任务的早期和后期学习阶段。与早期学习阶段组的晚期学习阶段组的背体纹状体中的更高强度，检测到具有自上而下串联MS的M / Z的离子，其鉴定为PEP-19 / PCP4。此外，突触体的质谱分析证实了突触在突触的PEP-19 / PCP4的存在。 PEP-19 / PCP4先前已被确定为机车学习中突触可塑性的关键决定因素。 O R调查结果将PEP-19 / PCP4功能扩展到前脑中的空间学习，并将MSI转发为有效和无偏见的研究策略，用于发现和识别学习，记忆和突触可塑性的分子机械。本文是题为：Maldi成像的特殊问题的一部分，由Corinna Henkel博士和Peter Hoffmann教授编辑

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