MO25, a conserved scaffold protein, activates the tumour suppressor LKB1 with the pseudokinase STRAD. MO25 also promotes the activities of the STE20-family kinases MST3, MST4, STK25, SPAK and OSR1. Zebrafish MO25 was purified and crystallized, and a crystal of zebrafish MO25 diffracted to 2.9 angstrom resolution and belonged to space group P3(2)21, with unit-cell parameters a = b = 156.665, c = 221.251 angstrom. The structure of zebrafish MO25 was determined by molecular replacement. It is constituted of seven helical repeats. Structural comparison indicates that the overall structures of zebrafish and human MO25 are very similar, suggesting that MO25 has conserved functions in zebrafish. This work provides a structural basis for further functional and evolutionary studies of MO25.
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机译：MO25，一种保守的支架蛋白，用假激酶STRAD激活肿瘤抑制因子LKB1。 MO25还促进STE20家族激酶MST3，MST4，STK25，SPAK和OSR1的活性。斑马鱼MO25进行了纯化和结晶，并且斑马鱼MO25的晶体衍射到2.9埃的分辨率，并属于空间群P3（2）21，其晶胞参数a = b = 156.665，c = 221.251埃。斑马鱼MO25的结构通过分子置换确定。它由七个螺旋重复组成。结构比较表明，斑马鱼的整体结构与人类MO25非常相似，表明MO25在斑马鱼中具有保守的功能。这项工作为MO25的进一步功能和进化研究提供了结构基础。
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