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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera
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Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera

机译：葡萄葡萄中UDP-葡萄糖醛酸：黄酮醇-3-O-葡萄糖醛酸转移酶（VvGT5）的结晶和初步X射线晶体分析

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摘要

Grapevine (Vitis vinifera) glycosyltransferase 5 (VvGT5) is a UDP-glucuronic acid: flavonol-3-O-glucuronosyltransferase that catalyses the 3-O-specific glucuronosylation of flavonols using UDP-glucuronic acid as a sugar donor to produce flavonol 3-O-glucosides, which are important bioactive phytochemicals. Recombinant VvGT5 expressed in Escherichia coli cells was purified and crystallized by the sitting-drop vapour-diffusion method. A full set of X-ray diffraction data was collected to 2.2 angstrom Bragg spacing from a single crystal using a synchrotron-radiation source. The crystal was hexagonal, belonging to space group P6(1)22, with unit-cell parameters a = b = 102.70, c = 535.92 angstrom. The initial phases were determined by the molecular-replacement method.
机译：葡萄（Vitis vinifera）糖基转移酶5（VvGT5）是UDP-葡糖醛酸：黄酮3-O-葡糖醛酸糖基转移酶，它使用UDP-葡糖醛酸作为糖供体来催化黄酮醇的3-O特异性葡糖醛酸糖基化反应，从而产生黄酮醇3-O -葡糖苷，其是重要的生物活性植物化学物质。通过坐滴蒸气扩散法纯化和结晶在大肠杆菌细胞中表达的重组VvGT5。使用同步辐射源，从单晶收集到完整的X射线衍射数据至2.2埃布拉格距离。晶体是六角形的，属于空间群P6（1）22，单位晶胞参数a = b = 102.70，c = 535.92埃。初始阶段通过分子置换法确定。

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