Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of 'loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose

Ohnuma Takayuki; Umemoto Naoyuki; Taira Toki; Fukamizo Tamo; Numata Tomoyuki

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Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of 'loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose

机译：壳聚糖与壳多糖的“无环”家族GH19几丁质酶活性位点突变体的结晶和初步X射线衍射分析

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The catalytic mechanism of family GH19 chitinases is not well understood owing to insufficient information regarding the three-dimensional structures of enzyme-substrate complexes. Here, the crystallization and preliminary X-ray diffraction analysis of a selenomethionine-labelled active-site mutant of 'loopless' family GH19 chitinase from the moss Bryum coronatum in complex with chitotetraose, (GlcNAc) 4, are reported. The crystals were grown using the vapour-diffusion method. They diffracted to 1.58 angstrom resolution using synchrotron radiation at the Photon Factory. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 74.5, b = 58.4, c = 48.1 angstrom, beta = 115.6 degrees. The asymmetric unit of the crystals is expected to contain one protein molecule, with a Matthews coefficient of 2.08 angstrom(3) Da(-1) and a solvent content of 41%.

机译：由于关于酶-底物复合物的三维结构的信息不足，对GH19几丁质酶的催化机理还没有很好的理解。在此，报道了来自青苔苔藓的'无环'家族GH19几丁质酶的硒代蛋氨酸标记的活性位点突变体的结晶和初步X射线衍射分析，该突变体与壳聚糖四糖（GlcNAc）4形成复合。使用蒸气扩散法生长晶体。他们在光子工厂使用同步加速器辐射衍射至1.58埃分辨率。晶体属于单斜晶空间群C2，其晶胞参数a = 74.5，b = 58.4，c = 48.1埃，β= 115.6度。晶体的不对称单元有望包含一个蛋白质分子，其马修斯系数为2.08埃（3）Da（-1），溶剂含量为41％。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第12期|共3页
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Ohnuma Takayuki; Umemoto Naoyuki; Taira Toki; Fukamizo Tamo; Numata Tomoyuki;
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2. Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of 'loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose [J] . Ohnuma Takayuki, Umemoto Naoyuki, Taira Toki, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第12期

机译：壳聚糖与壳多糖的“无环”家族GH19几丁质酶活性位点突变体的结晶和初步X射线衍射分析
3. Insertion of a Loop Structure into the "Loopless" GH19 Chitinase from Bryum coronatum [J] . Shoko Takenaka, Takayuki Ohnuma, Tamo Fukamizo Journal of Applied Glycoscience . 2017,第2期

机译：将环状结构插入冠状短杆菌的“无环” GH19几丁质酶中
4. Insertion of a Loop Structure into the “Loopless” GH19 Chitinase from Bryum coronatum [J] . Shoko Takenaka, Takayuki Ohnuma, Tamo Fukamizo Journal of Applied Glycoscience . 2017,第2期

机译：将环状结构插入冠状番茄的“无环” GH19几丁质酶中
5. X-ray Diffraction Analysis of the Effect of the NH4+: PO43- Molar Ratio on the Formation of Crystallized Struvite and Recovery Rate of Phosphorus [C] . Zongsheng Zhao, Xue Zou, Harry X. Zhang, The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议）论文集 . 2008

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7. Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of ‘loopless’ family GH19 chitinase from Bryum coronatum in a complex with chitotetraose [O] . Takayuki Ohnuma, Naoyuki Umemoto, Toki Taira, 2013

机译：壳聚糖与壳聚糖的无环 GH19几丁质酶活性位点突变体的结晶和初步X射线衍射分析
8. Interaction of di-N-acetylchitobiosyl moranoline with a family GH19 chitinase from moss, Bryum coronatum [O] . S. Shinya, A. Urasaki, T. Ohnuma, 2014

机译：Di-N-乙酰胆核苷酸Moranoline与来自苔藓，Bryum Coronatum的GH19 Chitinase的相互作用

Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of 'loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose

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