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首页> 外文期刊>Biofabrication >Electrochemical fabrication of a biomimetic elastin-containing bi-layered scaffold for vascular tissue engineering
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Electrochemical fabrication of a biomimetic elastin-containing bi-layered scaffold for vascular tissue engineering

机译：用于血管组织工程的含生物摩托蛋白的双层支架的电化学制备

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Biomimetic tissue-engineered vascular grafts (TEVGs) have immense potential to replace diseased small-diameter arteries (4 mm) for the treatment of cardiovascular diseases. However, biomimetic approaches developed thus far only partially recapitulate the physicochemical properties of the native vessel. While it is feasible to fabricate scaffolds that are compositionally similar to native vessels (collagen and insoluble elastic matrix) using freeze-drying, these scaffolds do not mimic the aligned topography of collagen and elastic fibers found in native vessels. Extrusion-based scaffolds exhibit anisotropic collagen orientation but these scaffolds are compositionally dissimilar (cannot incorporate insoluble elastic matrix). In this study, an electrochemical fabrication technique was employed to develop a biomimetic elastin-containing bi-layered collagen scaffold which is compositionally and structurally similar to native vessels and the effect of insoluble elastin incorporation on scaffold mechanics and smooth muscle cell (SMC) response was investigated. Further, the functionality of human umbilical vein endothelial cells (HUVECs) on the scaffold lumen surface was assessed via immunofluorescence. Results showed that incorporation of insoluble elastin maintained the overall collagen alignment within electrochemically aligned collagen (ELAC) fibers and this underlying aligned topography can direct cellular orientation. Ring test results showed that circumferential orientation of ELAC fibers significantly improved scaffold mechanics. Real-time PCR revealed that the expression of a-smooth muscle actin (Acta2) and myosin heavy chain (MyhII) was significantly higher on elastin containing scaffolds suggesting that the presence of insoluble elastin can promote contractility in SMCs. Further, mechanical properties of the scaffolds significantly improved post-culture indicating the presence of a mature cell-synthesized and remodeled matrix. Finally, HUVECs expressed functional markers on collagen lumen scaffolds. In conclusion, electrochemical fabrication is a viable method for the generation of a functional biomimetic TEVG with the potential to be used in bypass surgery.
机译：仿生组织工程化血管移植物（TEVGS）具有巨大的潜力，以取代患病的小直径动脉（＆ 4 mm）治疗心血管疾病。然而，迄今为止所开发的仿生方法仅局部重新延长天然容器的物理化学性质。虽然制造与天然容器（胶原蛋白和不溶性弹性基质）合成类似的支架是可行的，但是使用冷冻干燥，这些支架不会模拟胶原蛋白和在天然容器中发现的弹性纤维的对齐形貌。基于挤出的支架表现出各向异性胶原蛋白取向，但这些支架是组成不同的（不能包含不溶性弹性基质）。在该研究中，采用电化学制造技术进行含生物摩托蛋白的双层胶原支架，该胶囊是组成的，与天然血管合成和结构相似，并且不溶性弹性蛋白掺入对支架力学和平滑肌细胞（SMC）反应的影响调查。此外，通过免疫荧光评估支架腔表面上的人脐静脉内皮细胞（HUVEC）的功能。结果表明，不溶性弹性蛋白的掺入将电化学对准胶原（ELAC）纤维（ELAC）纤维内的总胶原取向保持，并且该下面的排列形貌可以直接细胞取向。环形试验结果表明，ELAC纤维的周向取向显着改善了支架力学。实时PCR显示，在含有支架上的弹性蛋白的含有支架上的表达显着提高，表明不溶性弹性蛋白的存在可以促进SMC中的收缩性。此外，支架的机械性能显着改善后培养后表明存在成熟细胞合成和改造的基质。最后，Huvecs表达了胶原蛋白腔骨架上的功能标志物。总之，电化学制造是一种用于产生功能性仿生TEVG的可行方法，其具有用于旁路手术的可能性。

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