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Genome-Wide Identification and Characterization of DIR Genes in Medicago truncatula

机译:Medicago Truncatula中的基因组鉴定与表征Dir基因

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摘要

Dirigent proteins (DIRs) are critically involved in the formation of lignans, a diverse and widely distributed class of secondary plant metabolites exhibiting interesting pharmacological activities and implicated in natural plant defense. However, no detailed information is available about DIR gene family in Medicago truncatula. In this study, a total of 45 DIR genes were identified in M. truncatula. DIR proteins have variability in sequence. Most MtDIR genes have no intron. All MtDIR proteins contain single dirigent domain. A large number of MtDIR genes were expanded via gene duplication, and 37 MtDIR genes were duplicated in tandem. Digital expression data showed that 40% MtDIR genes had a higher expression level in the root. Analysis of RNA-seq and microarray data indicated that more than 30% MtDIR genes were responsive to biotic and/or abiotic treatments. This study will facilitate further studies on DIR family and provide useful clues for functional validation ofDIR genes in higher plants.
机译:恶劣的蛋白质(DIR)批判性地参与木兰的形成,多样化和广泛分布的分布式的二级植物代谢物,其表现出有趣的药理学活动,并涉及天然植物防御。但是,在Medicago Truncatula的Dir Gene Family没有详细信息。在本研究中,在M. Truncatula中鉴定了总共45个DIR基因。 Dir蛋白依次具有可变性。大多数mtdir基因没有内含子。所有MTDIR蛋白质含有单个降噪结构域。通过基因重复扩增大量MTDIR基因,串联重复37个MTDIR基因。数字表达数据显示,40%的MTDIR基因在根系中具有更高的表达水平。 RNA-SEQ和微阵列数据的分析表明,超过30%的MTDIR基因对生物和/或非生物理学治疗敏感。本研究将促进DIR家族的进一步研究,并为高等植物中的DIR基因的功能验证提供有用的线索。

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