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首页> 外文期刊>Biochemical Genetics >Cloning, structure, and expression pattern of the P-450 aromatase gene in rice field eel (Monopterus albus).
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Cloning, structure, and expression pattern of the P-450 aromatase gene in rice field eel (Monopterus albus).

机译：水稻鳗鱼（Monopterus Albus）P-450芳族酶基因的克隆，结构和表达模式。

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摘要

We report the cloning, tissue expression, and structural analysis of the aromatase gene in the rice field eel (Monopterus albus). The ovary-derived cDNA (1,802 bp) has a 49 bp 5'-untranslated region (UTR), a 202 bp 3'-UTR, and a 1,551 bp open-reading frame, which encodes a protein of 517 amino acid residues with a predicted molecular weight of 58.2 kDa. The amino acid sequence alignment suggests that the rice field eel ovarian P-450 aromatase shares 63-80% identity with that of other fish species, reduced to 59-60% with brain-derived aromatases of other fishes and to 50% with human placenta aromatases. Between the 5' and 3' untranslated terminal regions, the rice field eel CYP19 gene contained seven introns at the same sites as in medaka and human but lacked an intron between the I-helix and the aromatase-specific conserved region. All introns conformed to the GT/AG rule. Sequence analysis of the 1,065 bp upstream of the translation start site revealed that the transcription initiation site was 51 bp upstream from the translation start site. This region had one estrogen receptor recognition half site (nt -62), five copies of an SRY/iSRY binding motif, a C/EBP (CCAAT enhancer binding protein) binding site (nt -751), chicken ovalbumin upstream promoter-transcription factor (nt -986) and GATA-2 (nt -186, -249) recognition sequences, but no binding sequence for steroidogenic factor-1 and the cAMP response element binding protein activating transcription factor family. In females, levels of relative expression were, in descending order, hypothalamus, pituitary, forebrain, ovary, and liver. In males, P450arom was detected only in the pituitary and the liver, with half the expression found in females. In fry, the P450arom expression level increased during development and was significantly higher in the brain than in the gonad.
机译：我们报道了稻田鳗鱼（Monopterus Albus）中芳香酶基因的克隆，组织表达和结构分析。卵巢衍生的cDNA（1,802bp）具有49bp 5' - 未旋转的区域（UTR），202bp 3'-UTR和1,551bp开放读数框架，其编码517个氨基酸残基的蛋白质预测分子量为58.2kDa。氨基酸序列对准表明，水稻场鳗鱼卵巢卵巢P-450芳族酸糖酶与其他鱼类的同一性63-80％，其其他鱼类的脑衍生芳香酶和人类胎盘的50％降至59-60％芳香酶。在5'和3'未翻译的终端区之间，米域EEL CYP19基因在与Medaka和人类的同一部位含有七个内部内部，但缺乏I-Helix和芳族酶特异性保守区域之间的内含子。所有内含器符合GT / AG规则。翻译开始遗址上游的1,065bp序列分析显示转录起始位点从翻译开始遗址上游51bp。该地区具有一个雌激素受体识别半场（NT -62），Sry / isry结合基序的五个拷贝，C / EBP（CCAAT增强剂结合蛋白）结合位点（NT -751），鸡卵嘌呤上游启动子转录因子（NT -986）和GATA-2（NT -186，-249）识别序列，但无菌因子-1的结合序列和CAMP反应元件结合蛋白激活转录因子家族。在女性中，相对表达的水平在降序，下丘脑，垂体，前脑，卵巢和肝脏。在雄性中，仅在垂体和肝脏中检测到p450玛莫，在雌性中发现的一半表达。在Fry中，P450Arom表达水平在发育过程中增加，大脑显着高于Gonad。

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  • 来源
    《Biochemical Genetics》 |2008年第6期|共14页
  • 作者

    Yu JH; Tang YK; Li JL;

  • 作者单位

    Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences Wuxi 214081 Jiangsu P;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
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