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TGF-β Negatively Regulates Mitf-E Expression and Canine Osteoclastogenesis

机译：TGF-β负调节MITF-E表达和犬骨核细胞发生

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摘要

With longevity, the prevalence of osteoporosis, which occurs when the activity of osteoclast surpasses that of osteoblasts, has increased in dogs. However, limited information is available on canine osteoclastogenesis. We herein described culture conditions to induce osteoclasts from canine bone marrow cells, and identified factors affecting canine osteoclastogenesis. Tartrate-resistant acid phosphatase-positive multinucleated cells were efficiently formed in a culture of bone marrow mononuclear cells with macrophage colony-stimulating factor (M-CSF 25?ng/mL) for 3?days and a subsequent culture in the presence of M-CSF (25?ng/mL) and soluble receptor activator of NF-κB ligand (RANKL 50?ng/mL) for 4?days. We previously reported in a murine cell system that gene induction of the E isoform of microphthalmia-associated transcription factor (Mitf-E) was required and sufficient for osteoclastogenesis, while transforming growth factor-β (TGF-β) enhanced RANKL-induced Mitf-E expression and osteoclastogenesis. Mitf-E expression also increased during RANKL-induced osteoclastogenesis in canine cells; however, TGF-β down-regulated Mitf-E expression and osteoclastogenesis, indicating a species-dependent response. The results of the present study show that, consistent with murine cells, M-CSF and soluble RANKL enable canine bone marrow cells to differentiate into osteoclasts, and Mitf-E expression is induced during osteoclastogenesis. However, the role of TGF-β in osteoclast formation is distinct between murine and canine cells, suggesting the necessity of analyses using canine cells to examine the factors affecting canine osteoclastogenesis.
机译：随着寿命的寿命，骨质疏松症的患病率发生在骨骨骨骨的活性超过成骨细胞的活动时，犬增加。但是，有限的信息可在犬骨骨壳发生。我们在本文中描述了培养条件，以诱导犬骨髓细胞的骨细胞，并确定影响犬骨骨细胞发生的因素。在巨噬细胞菌落刺激因子（M-CSF 25→Ng / mL）的骨髓单核细胞培养中有效地形成酒石酸耐酸性磷酸酶阳性多核细胞3？天和随后的培养物NF-κB配体的CSF（25〜Ng / mL）和可溶性受体活化剂（RANKL 50〜Ng / ml）4？天。我们以前在鼠细胞系统中报道，需要对微孔相关转录因子（MITF-e）的E同种型的基因诱导，并且足以用于骨质细胞发生，同时转化生长因子-β（TGF-β）增强的RANKL诱导的MITF- E表达和骨质增生发生。 MITF-E表达在犬细胞中的RANKL诱导的骨酸发生过程中也增加了;然而，TGF-β下调的MITF-e表达和骨质细胞发生，表明物种依赖性反应。本研究的结果表明，与鼠细胞，M-CSF和可溶性RANKL一致，使得犬骨髓细胞能够分化成骨质体，并且在骨质细胞发生期间诱导MITF-E表达。然而，TGF-β在破骨细胞形成中的作用在鼠和犬细胞之间是不同的，这表明使用犬细胞分析的必要性来检查影响犬骨细胞发生的因素。

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  • 来源
    《Biochemical Genetics》 |2018年第5期|共11页
  • 作者

    Kumiko Asai; Masaharu Hisasue; Fumie Shimokawa; Masayuki Funaba; Masaru Murakami;

  • 作者单位

    Laboratory of Molecular Biology Azabu University School of Veterinary Medicine;

    Laboratory of Small Animal Internal Medicine Azabu University School of Veterinary Medicine;

    Laboratory of Molecular Biology Azabu University School of Veterinary Medicine;

    Division of Applied Biosciences Kyoto University Graduate School of Agriculture;

    Laboratory of Molecular Biology Azabu University School of Veterinary Medicine;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    Osteoclast; TGF-β; Mitf-E; Dog;

    机译：骨核苷酸;tgf-β;mitf-e;狗;

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