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首页> 外文期刊>Biochemical Genetics >Omega-3 PUFA Alters the Expression Level but Not the Methylation Pattern of the WIF1 Gene Promoter in a Pancreatic Cancer Cell Line (MIA PaCa-2)
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Omega-3 PUFA Alters the Expression Level but Not the Methylation Pattern of the WIF1 Gene Promoter in a Pancreatic Cancer Cell Line (MIA PaCa-2)

机译：Omega-3 pufa改变了胰腺癌细胞系中WIF1基因启动子的表达水平（MIA Paca-2）

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摘要

Pancreatic cancer is the fourth leading cause of death in both males and females, with a 5-year relative survival rate of 8%. The Wnt signaling pathway has a significant role in the pathogenesis of many tumors, including those of pancreatic cancer. Hypermethylation of the Wnt inhibitory Factor-1 (WIF1) gene promoter have been detected in different types of cancer. In contrast, the anticancer effects of long-chain omega-3 PUFA (ALA) have been reported. Regarding its anticancer effects, in this study, we investigated the effects of various concentrations of omega-3 PUFA on expression level and promoter methylation of the WIF1 gene in MIA PaCa-2 cells in 24, 48, and 72h after treatment. MIA PaCa-2 cells were treated with different concentrations of omega-3 PUFA (25, 50, 100, 250, 500, and 1000 mu M). Cell viability assay was carried out followed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and methylation-specific PCR (MSP). This investigation suggested that dietary consumption of omega-3 PUFAs (250-1000 mu M) has a significant effect on the proliferation and WIF1 gene expression of the MIA PaCa-2 cancer cell line but no effect on the promoter methylation of this gene. Changes in promoter methylation were not observed in any of the treatments.
机译：胰腺癌是男性和女性死亡的第四个主要原因，5年相对生存率为8％。 WNT信号传导途径在许多肿瘤的发病机制中具有重要作用，包括胰腺癌的发病机制。 WNT抑制因子-1（WIF1）基因启动子的高甲基化已被检测在不同类型的癌症中。相比之下，报告了长链Omega-3 Pufa（ALA）的抗癌效应。关于其抗癌效应，在本研究中，我们研究了在治疗后24,48和72h的MIA Paca-2细胞中的各种浓度Omega-3 pufa对ω-2细胞的表达水平和启动子甲基化的影响。用不同浓度的ω-3 PUFA（25,50,100,250,500和1000μm）处理MIA Paca-2细胞。进行细胞活力测定，然后进行定量逆转录酶 - 聚合酶链反应（QRT-PCR）和特异性PCR（MSP）。该研究表明，ω-3 Pufas（250-1000亩）的膳食消费对MIA Paca-2癌细胞系的增殖和WiF1基因表达具有显着影响，对该基因的启动子甲基化没有影响。在任何治疗中未观察到启动子甲基化的变化。

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