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首页> 外文期刊>Biochemical Genetics >ISSR Characterization and Quantification of Purpurin and Alizarin in Rubia cordifolia L. Populations from India
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ISSR Characterization and Quantification of Purpurin and Alizarin in Rubia cordifolia L. Populations from India

机译：Rubia Cordifolia L.来自印度的紫癜和茜素的ISSR表征及定量

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摘要

Rubia cordifolia L., is an industrially viable medicinal crop and is widely exploited for the therapeutic potential of its bioactive metabolite, purpurin. The present investigation aimed to explore the chemotypic and molecular variability in seven wild populations of R. cordifolia from South Eastern Ghats region of India. Thirty-eight individuals were assessed for molecular fingerprinting (ISSR markers) and densitometric quantification of purpurin and alizarin. The populations of Yelagiri Hills and Shervaroy Hills contained the highest levels of alizarin (0.115 and 0.093%, respectively) while Pachamalai and Kolli Hills revealed the highest purpurin content (0.284 and 0.280%, respectively). Genetic diversity was generally higher in the same populations that produced higher metabolite content, with the exception of Pachamalai, suggesting a highly prioritized conservation concern. The study revealed a Nei's total gene diversity at species level of 0.266 and of 0.187 at population level, with an average population genetic differentiation of 0.28. No clear genetic or chemical structure was retrieved between the studied populations, with individuals from different locations clustering together, and no significant correlation was obtained between metabolites and genetic diversity or between these and the populations' geographic distances.
机译：Rubia Cordifolia L是一种工业活性的药物作物，广泛利用其生物活性代谢物，Purpurin的治疗潜力。目前的调查旨在探讨来自印度东南霍乱地区的七野生R.Str.Cordifolia的嗜型和分子变异。分子指纹（ISSR标记）和紫癜和茜素的密度计量评估了38个个体。 Yelagiri Hills和Shervaroy Hills的群体含有最高水平的茜素（分别为0.115和0.093％），而Pachamalai和Kolli Hills则显示出最高的紫癜含量（分别为0.284和0.280％）。在Pachamalai除外，遗传多样性在产生较高的代谢物含量的群体中通常更高，表明高优先考虑的保护问题。该研究揭示了NEI在物种水平0.266和人口水平下0.187的基因多样性，平均群体遗传分化为0.28。在研究的群体之间没有检测到不同位置聚集在一起的个体之间没有明确的遗传或化学结构，并且在代谢物和遗传多样性之间或这些和群体之间没有获得显着相关性。

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