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Investigation of Chinese Wolfberry (Lycium spp.) Germplasm by Restriction Site-Associated DNA Sequencing (RAD-seq)

机译:通过限制性位点相关DNA测序调查枸杞(枸杞SPP。)种质(RAD-SEQ)

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摘要

Chinese wolfberry (Lycium spp.) is an important edible and medicinal plant, with a long cultivation history. The genetic relationships among wild Lycium species and landraces have been unclear for a number of reasons, which has hindered the breeding of modern Chinese wolfberry cultivars. In this study, we collected 19 accessions of Chinese wolfberry germplasm, and constructed the genetic relationship based on RAD-seq markers. We obtained 30.32Gb of clean data, with the average value of each sample being 1.596Gb. The average mapping rate was 85.7%, and the average coverage depth was 6.76 X. The phylogeny results distinguished all accessions clearly. All the studied landraces shared their most recent common ancestor with L. barbarum, which indicated that L. barbarum may be involved in cultivation of these landraces. The relationship of some landraces, namely the Ningqi' series, Qingqi-1' and Mengqi-1,' has been supported by the phylogeny results, while the triploid wolfberry was shown to be based on a hybrid between Ningqi-1' and a tetraploid wolfberry. This study uncovered the genetic background of Chinese wolfberry, and developed the foundation for species classification, accession identification and protection, and the production of hybrid cultivars of wolfberry.
机译:中国枸杞(枸杞SPP)是一个重要的食用和药用植物,历史悠久。由于多种原因,野生枸杞种和体重增加区之间的遗传关系尚不清楚,这已经阻碍了现代枸杞品种的繁殖。在这项研究中,我们收集了19种枸杞种质的19种,基于Rad-SEQ标记构建了遗传关系。我们获得了30.32GB的清洁数据,每个样品的平均值为1.596GB。平均映射率为85.7%,平均覆盖深度为6.76 x。系统发育结果明确区分所有换乘。所有学习的样机都分享了与L. Barbarum的最新常见的祖先,这表明L. Barbarum可能参与培养这些实体的培养。一些实体的关系,即宁QI'系列,清奇-1'和孟汽-1,'已经得到了系统发育结果,而三倍体枸杞被证明基于宁QI-1'和四倍体之间的杂交物枸杞。本研究发现了中国枸杞的遗传背景,并开发了物种分类,加入鉴定和保护的基础,以及枸杞杂交品种的生产。

著录项

  • 来源
    《Biochemical Genetics》 |2018年第6期|共11页
  • 作者单位

    Qinghai Univ Qinghai Acad Agr &

    Forestry Xining 810016 Qinghai Peoples R China;

    Qinghai Gen Hlth Biotechnol Co LTD Xining 810003 Qinghai Peoples R China;

    Chinese Acad Forestry Res Inst Forestry Lab Cell Biol Xiangshan Rd Beijing 100091 Peoples R;

    Qinghai Univ Qinghai Acad Agr &

    Forestry Xining 810016 Qinghai Peoples R China;

    Qinghai Univ Qinghai Acad Agr &

    Forestry Xining 810016 Qinghai Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    Wolfberry; Goji; RAD-seq; Genetic background;

    机译:枸杞;goji;rad-seq;遗传背景;

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