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Isolation and characterization of the Cetn1 gene from tufted deer (Elaphodus cephalophus).

机译:簇绒鹿(Elaphodus Cephalophus)的分离与表征CETN1基因。

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摘要

The tufted deer, a species found only in China, has polymorphic sex chromosomes (2n = 46, 47, 48). Centrins are centrosome components in species from yeast to humans. Here, the Cetn1 gene was isolated from the testis cDNA library of tufted deer, and its expression pattern, number of gene copies, and gene structure were studied. To date, for unknown reasons, satisfactory genomic DNA of the tufted deer has not been obtained. In the present study, we extracted high molecular weight genomic DNA from tissues of the tufted deer. Results showed that genomic DNA was isolated successfully and could be used for Southern blotting of the Cetn1 gene. Centrin 1 transcripts were shown to exist in testis and the retina. It has no intron and only a single copy in the genome. Protein sequence analyses indicate that the C-terminal Ser-170, which was shown to be phosphorylated in human centrin, is not conserved except in sequences from the human, chimpanzee, and tufted deer.
机译:簇绒鹿,仅在中国发现的物种,具有多态性染色体(2n = 46,47,48)。 厘林斯是从酵母到人类的物种中的中心组分。 这里,从簇绒鹿的睾丸cDNA文库中分离CETN1基因,并研究了其表达模式,基因拷贝数和基因结构。 迄今为止,由于未知原因,尚未获得簇绒鹿的令人满意的基因组DNA。 在本研究中,我们从簇绒鹿的组织中提取了高分子量基因组DNA。 结果表明,基因组DNA成功分离,可用于CETN1基因的Southern印迹。 将Centrin 1转录物显示在睾丸和视网膜中存在。 它没有内含子,只有基因组中的单一副本。 蛋白质序列分析表明,除了来自人,黑猩猩和簇绒鹿的序列之外,视态末端Ser-170显示在人centrin中的磷酸化。

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