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首页> 外文期刊>Biochemical Genetics >Mapping, expression, and association study of the bovine PSMC1 gene.
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Mapping, expression, and association study of the bovine PSMC1 gene.

机译：牛PSMC1基因的映射，表达和结合研究。

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摘要

The 26S ATP-dependent protease is composed of a 20S catalytic proteasome and two PA700 regulatory modules; it plays a central role in many regulatory pathways, such as cell cycle regulation, differentiation, and apoptosis. The PA700 complex is composed of multiple subunits, including at least six related ATPases and approximately 15 non-ATPase polypeptides. PSMC1 (proteasome 26S subunit, ATPase, 1) is one of these ATPases. In this study, we amplified a fragment of 507 bp from intron 9 of the bovine PSMC1 gene and found a SNP (G/A) at position 216 in the PCR fragment. Genotyping of 138 animals from four beef breeds revealed that the average frequency for allele A (G-base) was 0.4271 (0.3269-0.5517); for allele B (A-base) it was 0.5729 (0.4483-0.6731). This SNP is significantly associated with average daily feed intake (P < 0.01), average daily gain, finishing average daily gain, body length, ratio of feed to meat, backfat thickness, and loin-muscle area (P < 0.05). Our experimental data showed that animals with an AA genotype have a significantly lower food intake, grow faster, are longer in the body, and have less backfat and bigger loin muscle; hence, their ratio of feed to meat is significantly lower. We believe that the PSMC1 SNP is a potential candidate marker for marker-assisted selection in these traits. We also found that the bovine PSMC1 gene was expressed mainly in lung, testis, and spleen. In addition, we mapped the bovine PSMC1 gene on BTA10 by an RH mapping method.
机译：26s依赖于ATP依赖性蛋白酶由20S催化蛋白酶组成和两种PA700调节模块组成;它在许多监管途径中起着核心作用，例如细胞周期调节，分化和细胞凋亡。 PA700复合物由多个亚基组成，包括至少六个相关的ATP酶和大约15个非ATP酶多肽。 PSMC1（蛋白酶体26s亚基，ATP酶，1）是这些ATP酶中的一种。在这项研究中，我们从牛PSMC1基因的内含子9中扩增了507bp的片段，发现PCR片段中的位置216处的SNP（G / A）。来自四只牛肉品种的138只动物的基因分型显示等位基因A（G碱）的平均频率为0.4271（0.3269-0.5517）;对于等位基因B（A碱）为0.5729（0.4483-0.6731）。该SNP与平均每日进料摄入（P <0.01），平均每日增益，整理平均每日增益，体长，饲料比例，肉，背部厚度和腰部肌肉区域的比例（P <0.05）。我们的实验数据显示，具有AA基因型的动物具有显着降低的食物摄入量，生长更快，身体更长，并且具有较少的背部和更大的腰部肌肉;因此，它们对肉的饲料比率显着降低。我们认为PSMC1 SNP是用于这些性状的标记辅助选择的潜在候选标记。我们还发现，牛PSMC1基因主要在肺，睾丸和脾脏中表达。此外，我们通过RH测绘方法映射了BTA10上的牛PSMC1基因。

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