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首页> 外文期刊>Biochemical Genetics >Modulation of Stimulator of Interferon Genes (STING) Expression by Interferon-γ in Human Keratinocytes
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Modulation of Stimulator of Interferon Genes (STING) Expression by Interferon-γ in Human Keratinocytes

机译：干扰素 - γ在人角蛋白细胞中干扰素基因刺激剂的调节

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摘要

Abstract Infection of microbial pathogen triggers the innate immune system, and the induction of interferons (IFNs) play a vital role in host antiviral response. Stimulator of interferon genes (STING) was identified as a crucial regulator of the DNA sensing pathway, and activates both nuclear factor-κB and interferon regulatory factor 3 transcription pathways to evoke IFNs production. In this study, we studied the upregulation of STING mRNA expression, induced by IFN-γ in human keratinocytes (HaCaT). STING promoter assays clarified that a gamma-activated sequence (GAS), located at ??7 to ??15-bp, is required for IFN-γ-upregulated promoter activity. Furthermore, an electrophoretic mobility shift assay showed that signal transducers and activators of transcription 1 (STAT1) attach to the GAS motif on the human STING promoter region. This indicates that IFN-γ/Janus kinases/STAT1 signaling is essential for the STING upregulation in human keratinocytes.
机译：摘要微生物病原体感染触发先天免疫系统，干扰素（IFNS）的诱导在宿主抗病毒反应中起着至关重要的作用。 干扰素基因的刺激剂（Sting）被鉴定为DNA感测途径的关键调节剂，并激活核因子-κB和干扰素调节因子3转录途径，以引起IFNS的生产。 在这项研究中，我们研究了在人角蛋白细胞（HACAT）中IFN-γ诱导的刺痛mRNA表达的上调。 STING启动子测定澄清说，IFN-γ-上调的启动子活性需要位于γ-活化的序列（气体），位于γ-γ-γ-γ-γ-β-15bp。 此外，电泳迁移率移位测定显示，转录1（Stat1）的信号换能器和转移剂连接到人体刺痛的启动子区域上的气体基序。 这表明IFN-γ/ Janus激酶/ Stat1信号传导对于人类角蛋白细胞的刺痛上调至关重要。

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  • 来源
    《Biochemical Genetics》 |2018年第2期|共10页
  • 作者

    Yohei Nishikawa; Yasushi Matsuzaki; Kazuyuki Kimura; Akiko Rokunohe; Hajime Nakano; Daisuke Sawamura;

  • 作者单位

    Department of Dermatology Hirosaki University Graduate School of Medicine;

    Department of Dermatology Hirosaki University Graduate School of Medicine;

    Department of Dermatology Hirosaki University Graduate School of Medicine;

    Department of Dermatology Hirosaki University Graduate School of Medicine;

    Department of Dermatology Hirosaki University Graduate School of Medicine;

    Department of Dermatology Hirosaki University Graduate School of Medicine;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    STING; IFN-γ; GAS; Keratinocyte;

    机译：刺痛;IFN-γ;气体;角质形成细胞;

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