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首页> 外文期刊>Biochemical Genetics >Morphological Characterization and Gene Expression Patterns for Melanin Pigmentation in Rex Rabbit
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Morphological Characterization and Gene Expression Patterns for Melanin Pigmentation in Rex Rabbit

机译：雷克斯兔黑色素色素沉着的形态学特征和基因表达模式

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摘要

Animal melanin has an important role in the formation of animal fur and skin, which is determined by its quantities, character, and distribution. To identify the effect of melanin on the formation of multi-colored Rex rabbits (Black, Chinchilla, Beaver, Protein cyan, Protein yellow, White), the structure of hair follicles and melanin content in multi-colored Rex rabbit skins were observed by Hematoxylin and Eosin (H&E) staining and melanin staining, respectively. The melanin granules were primarily found in the epidermis and hair follicle roots. The melanin content of skin was measured by extracting melanin from skin tissue. The results demonstrated that the melanin content was the highest in the skin of black Rex rabbit. Additionally, we measured the mRNA and protein expression levels of melanin-related key genes (MITF and TYR) in the skin of different hair color by quantitative real-time PCR and Wes assay, respectively. The results revealed that the mRNA expression levels in the skin of black Rex rabbit was highly expressed when as compared with other Rex rabbit skin (P 0.05). In summary, melanin deposition has important economic value, and the coat color of fur-bearing animals is partly determined by the melanin-related genes.
机译：动物黑素素在形成动物皮毛和皮肤中具有重要作用，这是由其数量，性格和分布决定的。为了鉴定黑色素对多色雷克斯兔形成的影响（黑色，栗鼠，海狸，蛋白质青色，蛋白质黄色，白色），通过苏木丁观察到多色Rex兔皮肤的毛囊和黑色素含量的结构和曙红（H＆E）染色和黑色素染色。黑色素颗粒主要在表皮和毛囊根中发现。通过从皮肤组织中提取黑色素来测量皮肤的黑色素含量。结果表明，黑色雷克斯兔皮肤中的黑色素含量最高。另外，我们通过定量实时PCR和WES测定分别测量了混合物相关的关键基因（MITF和TYR）的mRNA和蛋白表达水平。结果表明，与其他REX兔皮肤相比（P 0.05）相比，高表达黑色雷克斯兔皮肤中的mRNA表达水平。总之，黑色素沉积具有重要的经济价值，并且毛皮动物的涂层颜色部分由黑色素相关基因决定。

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