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首页> 外文期刊>Biochemical Genetics >Molecular characterization of high plant species using PCR with primers designed from consensus branch point signal sequences.
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Molecular characterization of high plant species using PCR with primers designed from consensus branch point signal sequences.

机译:用PCCR与共有分支点信号序列设计的引物的高植物物种的分子表征。

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摘要

A novel method is introduced for producing molecular markers in plants using single 15- to 18-mer PCR primers designed from the short conserved consensus branch point signal sequences and standard agarose gel electrophoresis. This method was tested on cultivated peanut and verified to give good fingerprinting results in other plant species (mango, banana, and longan). These single primers, designed from relatively conserved branch point signal sequences within gene introns, should be universal across other plant species. The method is rapid, simple, and efficient, and it requires no sequence information of the plant genome of interest. It could be used in conjunction with, or as a substitute for, conventional RAPD or ISSR techniques for applications including genetic diversity analysis, phylogenetic tree construction, and quantitative trait locus mapping. This technique provides a new way to develop molecular markers for assessing genetic diversity of germplasm in diverse species based on conserved branch point signal sequences.
机译:引入一种新的方法,用于使用从短保守共有分支点信号序列和标准琼脂糖凝胶电泳的单一15-至18-MEL PCR引物在植物中产生分子标记。该方法在培养的花生上进行了测试,并验证了其他植物物种(芒果,香蕉和龙眼)的良好指纹结果。这些单一引物,由基因内含子内的相对保守的分支点信号序列设计,应该是其他植物物种的普遍性。该方法快速,简单,高效,并且不需要植物基因组的序列信息。它可以与包括遗传多样性分析,系统发育树构造和定量性状轨迹映射的应用的替代,或作为替代,或作为替代,或作为替代,或作为替代,或作为替代,或作为常规RAPD或ISSR技术使用,包括遗传多样性分析,系统发育树施工和定量性状轨迹映射。该技术提供了一种新的方式来开发分子标记,用于评估基于保守分支点信号序列的不同物种种质的遗传多样性。

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