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首页> 外文期刊>Biochemical Pharmacology >Profiling Vaccinium macrocarpon components and metabolites in human urine and the urine ex-vivo effect on Candida albicans adhesion and biofilm-formation
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Profiling Vaccinium macrocarpon components and metabolites in human urine and the urine ex-vivo effect on Candida albicans adhesion and biofilm-formation

机译：分析疫苗丙酸雌激素组分和代谢物在人类尿液中和尿液对念珠菌粘附和生物膜形成的尿液效应

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摘要

The aim of this work was to profile, by using an HPLC-MS/MS method, cranberry compounds and metabolites found in human urine after ingestion of a highly standardized cranberry extract (Anthocran (R)). Two different strategies were adopted for the data analysis: a targeted and an untargeted approach. These strategies allowed the identification of 42 analytes including cranberry components, known metabolites and metabolites hitherto unreported in the literature, including six valerolactones/valeric acid derivatives whose presence in urine after cranberry consumption has never been described before. Absolute concentrations of 26 over 42 metabolites were obtained by using pure available standards. Urine collected at different time points after the last dosage of Anthocran (R) were tested on the reference strain C. albicans SC5314, a biofilm-forming strain. Fractions collected after 12 h were found to significantly reduce the adhesion and biofilm formation compared to the control (p < 0.05). A similar effect was then obtained by using Anthocran (TM) Phytosome (TM), the lecithin formulation containing 1/3 of standardized cranberry extract and formulated to enhance the absorption of the cranberry components. The urinary profile of cranberry components and metabolites in the urine fractions collected at 1 h, 6 h and 12 h after the last capsule intake were then reproduced by using the pure standards at the concentration ranges found in the urine fraction, and tested on C. albicans. Only the mixture mimicking the urinary fraction collected at 12 h and containing as main components, quercetin and 5-(3',4'-dihydroxyphenyl)-gamma-valerolactone was found effective thus confirming the ex-vivo results.
机译：通过使用HPLC-MS / MS方法，通过使用HPLC-MS / MS方法，在摄入高度标准化的蔓越莓提取物（花青群）后，在人尿中发现的蔓越莓化合物和代谢物。采用两种不同的策略来进行数据分析：目标和未确定的方法。这些策略允许鉴定42种分析物，包括蔓越莓组分，已知的代谢物和迄今为止在文献中未报告的代谢物，包括六个缬胺酮/ valeric酸衍生物，其在蔓越莓消耗后从未描述过尿液后的尿液。通过使用纯可用标准，获得了26克上42种代谢物的绝对浓度。在参考菌株C. albicans SC5314的参考菌株C. albicants菌株上测试在不同时间点的尿液在不同时间点。发现12小时后收集的馏分显着降低了与对照相比的粘附性和生物膜形成（P <0.05）。然后通过使用含有花青素（TM）的含有1/3标准化的蔓越莓提取物的卵磷脂制剂来获得类似的效果，并配制以增强蔓越莓组分的吸收。然后通过使用在尿液部分中发现的浓度范围的纯标准物在最后胶囊摄入后收集在1小时，6h和12h的尿液中的尿蛋白组分和代谢物中的尿液剖面和代谢物。老年人。只有在12小时内收集的尿液馏分并含有主要成分，槲皮素和5-（3'，4'-二羟基苯基）-Gamma-戊内酯的混合物被发现有效，从而证实了前体内结果。

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