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首页> 外文期刊>Cytokine >Induced heteroduplex genotyping of TNF-alpha, IL-1beta, IL-6 and IL-10 polymorphisms associated with transcriptional regulation.
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Induced heteroduplex genotyping of TNF-alpha, IL-1beta, IL-6 and IL-10 polymorphisms associated with transcriptional regulation.

机译:诱导与转录调控相关的TNF-α,IL-1beta,IL-6和IL-10多态性的异源双链基因分型。

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摘要

We describe the construction and use of 7 induced heteroduplex generators, reagents for the rapid and unequivocal genotyping of nucleotide sequence polymorphism in TNF-alpha, IL-1beta, IL-6 and IL-10. Polymorphisms detected are those previously associated with regulation of gene transcription: TNF-alpha positions -308 and -238; IL-1beta position +3953; IL-6 position -174; and IL-10 positions -1082, -819 and -592. The reagents were used for analysis of allele and haplotype frequencies in a population of healthy Caucasian volunteer blood donors. Copyright 1999 Academic Press.
机译:我们描述了7个诱导的异源双链生成器的构建和使用,这些试剂用于TNF-α,IL-1beta,IL-6和IL-10中核苷酸序列多态性的快速和明确的基因分型。检测到的多态性是先前与基因转录调控相关的那些:TNF-α位置-308和-238; IL-1beta位置+3953; IL-6位置-174; IL-10位置-1082,-819和-592。该试剂用于分析健康的白种人自愿献血者群体中的等位基因和单倍型频率。版权所有1999,学术出版社。

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