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首页> 外文期刊>Biochemistry >Optimization of Method for Human Sex Determination Using Peptidome Analysis of Teeth Enamel from Teeth of Different Biological Generation, Archeological Age, and Degrees of Taphonomic Preservation
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Optimization of Method for Human Sex Determination Using Peptidome Analysis of Teeth Enamel from Teeth of Different Biological Generation, Archeological Age, and Degrees of Taphonomic Preservation

机译：不同生物发电，考古时代牙齿牙釉质牙釉质肽分析的人性测定方法的优化

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摘要

Determination of biological sex to human remains is a fundamental requirement in anthropological, archeological, and forensic anthropological studies. Sex determination based on morphological criteria is significantly limited in the cases of juvenile remains and adult skeletons in a poor state of preservation. Regular attempts have been made to use alternative techniques to resolve this issue, including analysis of tooth enamel peptides by liquid chromatography/mass spectrometry. Optimization of this method involving acid etching of tooth enamel for 10 min followed by desalting of the products of etching on SDB-RPS StageTips microcolumns and analysis of desalted sample (1/3) by liquid chromatography/mass spectrometry allowed reliable sex determination to fossil remains within a wide range of archeological and biological ages without destructing analyzed teeth. Increasing the duration of enamel etching ensured a 2 to 3-fold increase in the total number of identified peptides and, more importantly, in the number of identified fragments of amelogenin Y isoform specific for male teeth, which facilitated reliable sex determination of fossil remains. The suggested technique was tested with 8 permanent and 15 deciduous teeth of different archaeological age and different degree of preservation. Two amelogenin Y-specific peptide sequences were identified. One of these peptides [SM(+15.99)IRPPYS)] was found in all male-derived samples without exception; the other peptide [IRPPYSS(+79.97)], which contained phosphorylated Ser66 residue, was found only in the enamel from deciduous teeth, which suggests that phosphorylation of Ser66 plays a role in the enamel formation in deciduous teeth.
机译：对人类生物性别的测定仍然是人类学，考古学和法医学研究中的基本要求。基于形态学标准的性别测定在少年遗骸和成年骨架中的保存状态差的情况下显着限制。已经定期尝试使用替代技术来解决这个问题，包括通过液相色谱/质谱法分析牙釉质肽。优化该方法涉及酸蚀刻牙釉质10分钟，然后通过液相色谱/质谱法测定蚀刻蚀刻的产物和脱盐样品（1/3）的分析允许对化石的可靠性测定在广泛的考古和生物年龄内没有破坏的牙齿。增加牙釉质蚀刻的持续时间确保了鉴定肽的总数增加了2至3倍，更重要的是，在阳牙的特异性鉴定的Amelogen y同种型的数量中，这有助于化石的可靠性测定。建议的技术用8个永久性和15个落叶的牙齿进行了测试，不同的考古年龄和不同的保存程度。鉴定了两种Amelogen y特异性肽序列。所有雄性衍生的样品中发现其中一种肽[SM（+15.99）IRPPYS）没有异常;含有磷酸化的Ser66残基的另一个肽[Irppyss（+79.97）]仅在落叶牙牙釉质中发现，这表明Ser66的磷酸化在落叶牙釉质中起作用。

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