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Multiparametric Time-Correlated Single Photon Counting Luminescence Microscopy

机译:多次时间相关的单光子计数发光显微镜

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摘要

Classic time-correlated single photon counting (TCSPC) technique involves detection of single photons of a periodic optical signal, registration of the photon arrival time in respect to the reference pulse, and construction of photon distribution with regard to the detection times. This technique achieves extremely high time resolution and near-ideal detection efficiency. Modern TCSPC is multi-dimensional, i.e., in addition to the photon arrival time relative to the excitation pulse, spatial coordinates within the image area, wavelength, time from the start of the experiment, and many other parameters are determined for each photon. Hence, the multi-dimensional TCSPC allows generation of photon distributions over these parameters. This review describes both classic and multi-dimensional types of TCSPC microscopy and their application for fluorescence lifetime imaging in different areas of biological studies.
机译:经典时间相关的单光子计数(TCSPC)技术涉及检测周期光信号的单个光子,对参考脉冲的光子到达时间的登记,以及关于检测时间的光子分布的构造。 该技术实现了极高的时间分辨率和近乎理想的检测效率。 现代TCSPC是多维的,即除了相对于激励脉冲相对于激励脉冲的光子到达时间,图像区域内的空间坐标,波长,从实验开始的时间,以及每个光子确定许多其他参数。 因此,多维TCSPC允许在这些参数上产生光子分布。 本综述描述了TCSPC显微镜的经典和多维类型及其在生物学研究不同领域荧光寿命成像的应用。

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