Aim23p Interacts with the Yeast Mitochondrial Ribosomal Small Subunit

Chicherin I. V.; Zinina V. V.; Levitskiy S. A.; Serebryakova M. V.; Kamenski P. A.

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Aim23p Interacts with the Yeast Mitochondrial Ribosomal Small Subunit

机译：AIM23P与酵母线粒体核糖体小亚基相互作用

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Protein synthesis in mitochondria is generally organized in a bacterial-like manner but, at the same time, possesses several unique traits. Translation initiation in mitochondria is regulated by two protein factors, mtIF2 and mtIF3. Previously we demonstrated that Saccharomyces cerevisiae Aim23 protein is an ortholog of IF3 in budding yeast. However, the data on the interactions between Aim23p and other proteins are limited. Here, we demonstrated that Aim23p interacts with the yeast mitochondrial ribosomal small subunit both in vivo and in vitro using co-immunoprecipitation and density gradient sedimentation.

机译：线粒体中的蛋白质合成通常以细菌的方式组织，但同时具有几种独特的特征。线粒体中的翻译引发由两种蛋白质因子，mtif2和mtif3调节。以前我们证明酿酒酵母酿酒酵母AIM23蛋白是IF3在萌芽酵母中的垂直。然而，关于AIM23P和其他蛋白质之间的相互作用的数据有限。在这里，我们证明AIM23P使用共免疫沉淀和密度梯度沉降，与酵母线粒体核糖体小亚基与酵母线粒体核糖体小亚基相互作用。

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来源
《Biochemistry》 |2019年第1期|共7页
作者
Chicherin I. V.; Zinina V. V.; Levitskiy S. A.; Serebryakova M. V.; Kamenski P. A.;
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作者单位

Lomonosov Moscow State Univ Fac Biol Moscow 119991 Russia;

Lomonosov Moscow State Univ Fac Biol Moscow 119991 Russia;

Lomonosov Moscow State Univ Fac Biol Moscow 119991 Russia;

Lomonosov Moscow State Univ Belozersky Inst Physicochem Biol Moscow 119991 Russia;

Lomonosov Moscow State Univ Fac Biol Moscow 119991 Russia;

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原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
mitochondria; translation; ribosomes; initiation factor; Aim23p;

机译：线粒体;翻译;核糖体;启动因子;AIM23P;

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专利

1. Aim23p Interacts with the Yeast Mitochondrial Ribosomal Small Subunit [J] . Chicherin I. V., Zinina V. V., Levitskiy S. A., Biochemistry . 2019,第1期

机译：AIM23P与酵母线粒体核糖体小亚基相互作用
2. The fission yeast RPA21 subunit of RNA polymerase I: an evolutionarily conserved subunit interacting with ribosomal DNA (rDNA) transcription factor Rrn3p for recruitment to rDNA promoter [J] . Kaori Nakagawa, Koji Hisatake, Masami Muramatsu, Genes & Genetic Systems . 2002,第3期

机译：RNA聚合酶I的裂变酵母RPA21亚基：与核糖体DNA（rDNA）转录因子Rrn3p相互作用的进化保守亚基，可募集到rDNA启动子
3. Human C4orf14 interacts with the mitochondrial nucleoid and is involved in the biogenesis of the small mitochondrial ribosomal subunit. [J] . He J, Cooper HM, Reyes A, Nucleic Acids Research . 2012,第13期

机译：人C4orf14与线粒体的核苷相互作用，并参与小线粒体核糖体亚基的生物发生。
4. Comparative modelling and virtual screening to discover potential competitive inhibitors targeting the 30s ribosomal subunit S2 and S9 in Acinetobacter baumannii [C] . Priyansh Srivastava, Rajesh Pal, Gauri Misra International Conference on Bioinformatics and Systems Biology . 2018

机译：比较建模和虚拟筛选，以发现针对鲍曼不动杆菌中30s核糖体亚基S2和S9的潜在竞争性抑制剂
5. Molecular systematics of Lipotyphla (Mammalia): Evidence from the mitochondrial genes 12S ribosomal RNA (12S rRNA) and nicotinamide adenine dinucleotide dehydrogenase subunit 2 (ND2). [D] . Emerson, Ginny Leigh. 2002

机译：Lipotyphla（哺乳动物）的分子系统：来自线粒体基因12S核糖体RNA（12S rRNA）和烟酰胺腺嘌呤二核苷酸脱氢酶亚基2（ND2）的证据。
6. Human C4orf14 interacts with the mitochondrial nucleoid and is involved in the biogenesis of the small mitochondrial ribosomal subunit [O] . J. He, H. M. Cooper, A. Reyes, 2012

机译：人类C4orf14与线粒体核苷酸相互作用并参与小线粒体核糖体亚基的生物发生
7. The fission yeast RPA21 subunit of RNA polymerase I: An evolutionarily conserved subunit interacting with ribosomal DNA (rDNA) transcription factor Rrn3p for recruitment to rDNA promoter. [O] . Yukiko Imazawa, Koji Hisatake, Kaori Nakagawa, 2002

机译：RNA聚合酶I的裂变酵母RPA21亚基I：一种与核糖体DNA（RDNA）转录因子RRN3P募集到RDNA启动子的进化保守亚基。

Aim23p Interacts with the Yeast Mitochondrial Ribosomal Small Subunit

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