...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >A Novel Fluorescent GFP Chromophore Analog-Based Dye for Quantitative PCR
【24h】

A Novel Fluorescent GFP Chromophore Analog-Based Dye for Quantitative PCR

机译：一种用于定量PCR的新型荧光GFP发色团模拟染料

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This is the first report describing the possibility of using a green fluorescent protein chromophore synthetic analog, P-HOBDI-BF~(2), as a fluorescent dye for a linear hydrolysis probe used in qPCR. The study was carried out on a system for detection of the plant pathogenic fungus Fusarium avenaceum using a plasmid containing translation elongation factor 1α fragment as a template. To estimate fluorogenic properties of P-HOBDI-BF~(2), 6-FAM-and BDP-FL-labeled probes were used. It was demonstrated that a synthetic dye based on the P-HOBDI-BF~(2)chromophore can be used for labeling hydrolysis probes for qPCR, but fluorescence increase levels for P-HOBDI-BF~(2)-labeled probes were slightly lower than those for 6-FAM-labeled ones. At the same time, the sensitivity of P-HOBDI-BF~(2)-based assays remained high, and this fact together with acceptable fluorescence levels suggests that this dye can be considered as an efficient alternative for reporters traditionally used for fluorescence detection in the FAM channel.
机译：这是第一报告，描述使用绿色荧光蛋白发色团合成模拟，P-HOBDI-BF〜（2）的可能性，作为用于QPCR的线性水解探针的荧光染料。该研究在用于检测植物致病真菌镰刀菌植物的系统上进行，所述植物致病真菌镰刀菌纤维化纤维化纤维化纤维化纤维化纤维化纤维化纤维化纤维化纤维化纤维质植物为模板作为模板。为了估计P-HOBDI-BF〜（2）的荧光性能，使用6-FAM和BDP-FL标记的探针。结果证明，基于P-HOBDI-BF〜（2）发色团的合成染料可用于标记QPCR的水解探针，但P-HOBDI-BF〜（2）标记探针的荧光增加水平略低比那些6-FAM标记的人。同时，基于P-HOBDI-BF〜（2）的测定的敏感性仍然很高，并且该事实与可接受的荧光水平一起表明，该染料可以被认为是传统上用于荧光检测的记者的有效替代方案FAM频道。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号