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首页> 外文期刊>Biochemistry >Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549
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Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549

机译：K32荚膜多糖结构的阐明，K132基因的致胰杆菌Baumannii Luh5549的表征

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摘要

Capsular polysaccharide (CPS), isolated from Acinetobacter baumannii LUH5549 carrying the KL32 capsule biosynthesis gene cluster, was studied by sugar analysis, Smith degradation, and one- and two-dimensional H-1 and C-13 NMR spectroscopy. The K32 CPS was found to be composed of branched pentasaccharide repeats (K units) containing two residues of beta-D-GalpNAc and one residue of beta-D-GlcpA (beta-D-glucuronic acid) in the main chain and one residue each of beta-D-Glcp and alpha-D-GlcpNAc in the disaccharide side chain. Consistent with the established CPS structure, the KL32 gene cluster includes genes for a UDP-glucose 6-dehydrogenase (Ugd3) responsible for D-GlcA synthesis and four glyco- syltransferases that were assigned to specific linkages. Genes encoding an acetyltransferase and an unknown protein prod- uct were not involved in CPS biosynthesis. Whilst the KL32 gene cluster has previously been found in the global clone 2 (GC2) lineage, LUH5549 belongs to the sequence type ST354, thus demonstrating horizontal gene transfer between these lineages.
机译：通过糖分析，史密斯降解和一尺寸的H-1和C-13 NMR光谱研究了携带KL32胶囊生物合成基因簇的患有KL32胶囊生物合成基因簇的患有KL32胶囊生物合成基因簇的囊状多糖（CPS）。发现K32CPS由含有两种残留的β-D-加仑的重复（K单位）和主链中的β-D-Glcpa（β-D-葡萄糖醛酸）的一个残留物组成，每种残留物β-D-GLCP和α-D-GLCPNAc在二糖侧链中。与所建立的CPS结构一致，KL32基因簇包括用于D-Glca合成的UDP-葡萄糖6-脱氢酶（UGD3）的基因，其分配给特定键的四种甘油三烷烃。编码乙酰转移酶和未知蛋白质产品的基因没有参与CPS生物合成。虽然在全局克隆2（GC2）谱系中发现了KL32基因簇，但是LuH5549属于ST354的序列类型，因此在这些谱系之间展示了水平基因转移。

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