Binding of nuclear proteins to the negative regulatory element of the IL-2 gene in lymphocytes from rheumatic patients.

Flescher E; Vela Roch-N; Talal N; Dang H

首页> 外文期刊>Cytokine >Binding of nuclear proteins to the negative regulatory element of the IL-2 gene in lymphocytes from rheumatic patients.

【24h】

Binding of nuclear proteins to the negative regulatory element of the IL-2 gene in lymphocytes from rheumatic patients.

机译：风湿病患者淋巴细胞中核蛋白与IL-2基因负调控元件的结合。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

T lymphocytes from several autoimmune diseases including rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) exhibit deficient mitogenic response in terms of proliferation and IL-2 production. The expression of the IL-2 gene is regulated by various transcription factors. One of these factors suppresses IL-2 expression and binds to the negative responsive element in the IL-2 gene 5' flanking region (NRE-A). The authors hypothesized that the decreased production of IL-2 by T cells from RA and SLE patients is at least partially caused by high expression of the NRE-A binding protein. To test this hypothesis T cells from healthy donors and patients with RA and SLE were stimulated. Using the electrophoretic mobility shift assay we detected NRE-A DNA-binding proteins in the nuclei of the stimulated cells. No difference was found between NRE-A DNA binding in nuclear extracts of T cells taken from healthy donors and those taken from patients. The specificity of the DNA-protein interactions was ascertained through the use of unlabeled DNA competitors. No correlation was found between DNA-binding and the patients' disease duration or medication. In conclusion, decreased IL-2 biosynthesis by T lymphocytes from RA and SLE patients can not be explained by abnormal expression of the NRE-A DNA-binding protein. Copyright 1999 Academic Press.

机译：来自几种自身免疫性疾病的T淋巴细胞，包括类风湿性关节炎（RA）和系统性红斑狼疮（SLE），在增殖和IL-2产生方面均显示出不足的促有丝分裂反应。 IL-2基因的表达受多种转录因子调控。这些因素之一抑制IL-2表达，并与IL-2基因5'侧翼区（NRE-A）中的负响应元件结合。作者假设，RA和SLE患者的T细胞产生的IL-2降低至少部分是由NRE-A结合蛋白的高表达引起的。为了检验该假设，刺激了来自健康供体以及RA和SLE患者的T细胞。使用电泳迁移率变动分析，我们在受激细胞的细胞核中检测到NRE-A DNA结合蛋白。从健康供体和患者体内提取的T细胞核提取物中的NRE-A DNA结合没有发现差异。 DNA-蛋白质相互作用的特异性通过使用未标记的DNA竞争剂来确定。在DNA结合与患者的病程或药物治疗之间未发现相关性。总之，不能通过NRE-A DNA结合蛋白的异常表达来解释RA和SLE患者T淋巴细胞的IL-2生物合成减少。版权所有1999，学术出版社。

著录项

来源
《Cytokine》 |1999年第3期|共5页
作者
Flescher E; Vela Roch-N; Talal N; Dang H;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
DNA-Binding Proteins; Interleukin-2; Nuclear Proteins; DNA结合蛋白质类; 白细胞介素2; 核蛋白质类;

机译：DNA-Binding Proteins;Interleukin-2;Nuclear Proteins;DNA结合蛋白质类;白细胞介素2;核蛋白质类;

相似文献

外文文献
中文文献
专利

1. Binding of nuclear proteins to the negative regulatory element of the IL-2 gene in lymphocytes from rheumatic patients. [J] . Flescher E, Vela Roch-N, Talal N, Cytokine . 1999,第3期

机译：风湿病患者淋巴细胞中核蛋白与IL-2基因负调控元件的结合。
2. Protein kinase C-theta participates in the activation of cyclic AMP-responsive element-binding protein and its subsequent binding to the -180 site of the IL-2 promoter in normal human T lymphocytes. [J] . Solomou EE, Juang YT, Tsokos GC The Journal of Immunology: Official Journal of the American Association of Immunologists . 2001,第9期

机译：蛋白激酶C-theta参与环状AMP应答元件结合蛋白的活化及其随后与正常人T淋巴细胞中IL-2启动子的-180位结合。
3. ProteinKinase C-#theta# Participates in the activation f cyclic AMP-responsive element-binding proteinand its subsequent binding to the -180 Site of the IL-2 promoter in Normal Human T lymphocytes [J] . Elena E.Solomou, Yuang-Taung Juang, George C.Tsokos The Journal of Immunology: Official Journal of the American Association of Immunologists . 2001,第9期

机译：ProteinKinase C-＃theta＃参与激活环状AMP反应元件结合蛋白及其随后与正常人T淋巴细胞IL-2启动子的-180位点的结合
4. FoxO1 Inhibits Sterol Regulatory Element-binding Protein-1c (SREBP-1c) Gene Expression via Transcription Factors SREBP-1c and LXR in Goat Mammary Epithelial Cells [C] . Q.Y.He, J.Luo, J.Wu The 6th International Symposium on Dairy Cow Nutrition and Milk Quality（第六届“奶牛营养与牛奶质量”国际研讨会）论文集 . -1

机译：FOXO1通过转录因子Srebp-1C和LXR抑制甾醇调节元素结合蛋白-1C（Sreb-1c）基因表达，山羊乳腺上皮细胞
5. Regulatory elements in the Staphylococcus aureus protein A (Spa) gene promoter and characterization of SarS binding to the spa promoter [D] . Gao, Jinxin. 2004

机译：金黄色葡萄球菌蛋白A（Spa）基因启动子中的调控元件以及与Spa启动子结合的SarS的特征
6. Distinct nuclear proteins competing for an overlapping sequence of cyclic adenosine monophosphate and negative regulatory elements regulate tissue-specific mouse renin gene expression. [O] . M Horiuchi, R E Pratt, N Nakamura, 1993

机译：相互竞争的环状核苷单磷酸和负调控元件的重叠序列的不同核蛋白调节组织特异性小鼠肾素基因表达。
7. Yin Yang 1 Protein Negatively Regulates High-Density Lipoprotein Receptor Gene Transcription by Disrupting Binding of Sterol Regulatory Element Binding Protein to the Sterol Regulatory Element1 [O] . Wendy Shea-Eaton, Dayami Lopez, Mark P. McLean 2001

机译：尹阳1蛋白通过破坏甾醇调节元素结合蛋白与甾醇调节元素1的结合来调节高密度脂蛋白受体基因转录
8. Evidence for the Involvement of Three Distinct Signals in the Induction of IL-2 Gene Expression in Human T Lymphocytes. [R] . June, C. H., Ledbetter, J. A., Lindsten, T., 1989

机译：三种不同信号参与诱导人T淋巴细胞IL-2基因表达的证据。

Binding of nuclear proteins to the negative regulatory element of the IL-2 gene in lymphocytes from rheumatic patients.

摘要

著录项

相似文献

相关主题

期刊订阅