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首页> 外文期刊>Cytokine >Expression of icb-1 gene is interferon-gamma inducible in breast and ovarian cancer cell lines and affects the IFNgamma-response of SK-OV-3 ovarian cancer cells.
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Expression of icb-1 gene is interferon-gamma inducible in breast and ovarian cancer cell lines and affects the IFNgamma-response of SK-OV-3 ovarian cancer cells.

机译：icb-1基因的表达在乳腺癌和卵巢癌细胞系中可被干扰素-γ诱导，并影响SK-OV-3卵巢癌细胞的IFN-γ反应。

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Icb-1 (C1orf38) is a human gene initially described to be involved in in vitro differentiation processes of endometrial adenocarcinoma and leukemia cells. In this study, we examined the effect of interferon-gamma on icb-1alpha and beta mRNA levels in human cell lines derived from breast cancer and gynecological malignancies. Furthermore, we intended to approach icb-1 gene function by means of RNA interference (RNAi) to analyze the effect of an icb-1 knockdown on human cancer cells in vitro. Three breast cancer cell lines (MCF-7, SK-BR-3, MDA-MB-231), three ovarian cancer cell lines (SK-OV-3, OVCAR-3 and BG-1) and the endometrial adenocarcinoma cell line HEC-1-A were treated with interferon gamma and the transcript levels of icb-1 isoforms alpha and beta were assessed by means of semiquantitative real-time RT-PCR. Our data demonstrates an interferon-gamma triggered upregulation of icb-1alpha mRNA levels in all breast cancer cell lines and an increase of icb-1beta mRNA in MDA-MB-231 cells. The strongest (about 10-fold) increase of icb-1alpha and beta mRNA after treatment with interferon-gamma was observed in ovarian cancer cell line SK-OV-3. Additionally, our data demonstrates the success of a siRNA-mediated knockdown of icb-1alpha and beta mRNA levels, which resulted in a significant increase of the antiproliferative interferon-gamma effect on SK-OV-3 cells. In conclusion, we report identification of the novel interferon-gamma inducible gene icb-1 which is able to affect the response of ovarian cancer cells to this cytokine.
机译：Icb-1（C1orf38）是人类基因，最初被描述为参与子宫内膜腺癌和白血病细胞的体外分化过程。在这项研究中，我们检查了干扰素-γ对源自乳腺癌和妇科恶性肿瘤的人细胞系中icb-1alpha和βmRNA水平的影响。此外，我们打算通过RNA干扰（RNAi）来研究icb-1基因的功能，以分析icb-1敲低对体外人癌细胞的影响。三种乳腺癌细胞系（MCF-7，SK-BR-3，MDA-MB-231），三种卵巢癌细胞系（SK-OV-3，OVCAR-3和BG-1）和子宫内膜腺癌细胞系HEC用干扰素γ处理-1-A，并通过半定量实时RT-PCR评估icb-1同工型α和β的转录水平。我们的数据表明，干扰素-γ触发了所有乳腺癌细胞系中icb-1alpha mRNA水平的上调以及MDA-MB-231细胞中icb-1beta mRNA的增加。在卵巢癌细胞系SK-OV-3中观察到用干扰素-γ处理后icb-1alpha和beta mRNA的增加最强（约10倍）。此外，我们的数据证明了siRNA介导的icb-1alpha和beta mRNA水平敲低的成功，这导致抗增殖干扰素-γ对SK-OV-3细胞的作用显着增加。总之，我们报告了新型干扰素-γ诱导型基因icb-1的鉴定，该基因能够影响卵巢癌细胞对此细胞因子的反应。

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