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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Expression at 279 K, purification, crystallization and preliminary X-ray crystallographic analysis of a novel cold-active beta-1,4-D-mannanase from the Antarctic springtail Cryptopygus antarcticus
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Expression at 279 K, purification, crystallization and preliminary X-ray crystallographic analysis of a novel cold-active beta-1,4-D-mannanase from the Antarctic springtail Cryptopygus antarcticus

机译：在279 K表达，纯化，结晶和初步的X射线晶体学分析的新型冷活性β-1,4-D-甘露聚糖酶从南极跳马Cryptopygus antarcticus

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摘要

The CaMan gene product from Cryptopygus antarcticus, which belongs to the glycoside hydrolase family 5 type beta-1,4-D-mannanases, has been crystallized using a precipitant solution consisting of 0.1 M Tris-HCl pH 8.5, 25%(w/v) polyethylene glycol 3350 by the microbatch crystallization method at 295 K. The CaMan protein crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 73.40, b = 83.81, c = 163.55 angstrom. Assuming the presence of two molecules in the asymmetric unit, the solvent content was estimated to be about 61.29%. CaMan-mannopentaose (M5) complex crystals that were isomorphous to the CaMan crystals were obtained using the same mother liquor containing 1 mM M5.
机译：来自南极隐孢子虫的CaMan基因产物属于糖苷水解酶家族5型β-1,4-D-甘露聚糖酶，已使用由0.1 M Tris-HCl pH 8.5、25％（w / v）组成的沉淀剂溶液进行了结晶。 ）在295 K下通过微分批结晶法制备的聚乙二醇3350。CaMan蛋白晶体属于空间群P2（1）2（1）2（1），单位细胞参数a = 73.40，b = 83.81，c = 163.55埃。假设不对称单元中存在两个分子，则溶剂含量估计为约61.29％。使用相同的含有1 mM M5的母液，获得与CaMan晶体同晶的CaMan-甘露半乳糖（M5）复合晶体。

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