Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6-FCR3 of the Plasmodium falciparum VAR2CSA protein

Vuchelen Anneleen; Pardon Els; Steyaert Jan; Gamain Benoit; Loris Remy; van Nuland Nico A. J.; Ramboarina Stephanie

首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6-FCR3 of the Plasmodium falciparum VAR2CSA protein

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Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6-FCR3 of the Plasmodium falciparum VAR2CSA protein

机译：针对恶性疟原虫VAR2CSA蛋白的达菲结合样（DBL）域DBL6-FCR3的两个纳米抗体的生产，结晶和X射线衍射分析

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The VAR2CSA protein has been closely associated with pregnancy-associated malaria and is recognized as the main adhesin exposed on the surface of Plasmodium falciparum-infected erythrocytes. Chondroitin sulfate A was identified as the main host receptor in the placenta. Single-domain heavy-chain camelid antibodies, more commonly called nanobodies, were selected and produced against the DBL6-FCR3 domain of VAR2CSA. Crystals of two specific nanobodies, Nb2907 and Nb2919, identified as strong binders to DBL6-FCR3 and the full-length VAR2CSA exposed on the surface of FCR3 P. falciparum-infected erythrocytes, were obtained. Crystals of Nb2907 diffract to 2.45 angstrom resolution and belong to space group C2 with unit-cell parameters a = 136.1, b = 78.5, c = 103.4 angstrom, = 118.8 degrees, whereas Nb2919 crystals diffract to 2.15 angstrom resolution and belong to space group P43212 with unit-cell parameters a = b = 62.7, c = 167.2 angstrom.

机译：VAR2CSA蛋白与妊娠相关的疟疾密切相关，被认为是暴露于恶性疟原虫感染的红细胞表面的主要粘附素。硫酸软骨素A被确认为胎盘中的主要宿主受体。针对VAR2CSA的DBL6-FCR3结构域，选择并产生了单结构域重链骆驼科动物抗体（通常称为纳米抗体）。获得了两个特定的纳米抗体Nb2907和Nb2919的晶体，它们被确定是DBL6-FCR3和暴露于FCR3恶性疟原虫感染的红细胞表面的全长VAR2CSA的强结合剂。 Nb2907晶体衍射到2.45埃分辨率，并属于C2空间群，单位像元参数为a = 136.1，b = 78.5，c = 103.4埃，= 118.8度，而Nb2919晶体衍射到2.15埃分辨率，属于空间群P43212晶胞参数a = b = 62.7，c = 167.2埃。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第3期|共5页
作者
Vuchelen Anneleen; Pardon Els; Steyaert Jan; Gamain Benoit; Loris Remy; van Nuland Nico A. J.; Ramboarina Stephanie;
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正文语种 eng
中图分类晶体学;
关键词
pregnancy-associated malaria; VAR2CSA; DBL domain; nanobody;

机译：妊娠相关疟疾;VAR2CSA;DBL结构域;纳米抗体;

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1. Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6-FCR3 of the Plasmodium falciparum VAR2CSA protein [J] . Vuchelen Anneleen, Pardon Els, Steyaert Jan, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第3期

机译：针对恶性疟原虫VAR2CSA蛋白的达菲结合样（DBL）域DBL6-FCR3的两个纳米抗体的生产，结晶和X射线衍射分析
2. Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6 epsilon-FCR3 of the Plasmodium falciparum VAR2CSA protein (vol 69, pg 270, 2013) [J] . Vuchelen Anneleen, Pardon Els, Steyaert Jan, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第11期

机译：针对恶性疟原虫VAR2CSA蛋白Duffy结合样（DBL）域DBL6 epsilon-FCR3的两个纳米抗体的生产，结晶和X射线衍射分析（第69卷，第270页，2013）
3. Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid nanobody [J] . Van Son Nguyen, Spinelli Silvia, Desmyter Aline, Acta crystallographica. Section F, Structural biology communications . 2015,第3期

机译：TssM T6SS蛋白片段与骆驼科动物纳米抗体之间的复合物的产生，结晶和X射线衍射分析
4. Molecular Interactions Between PFCCP Multidomain Adhesion Proteins During Gametogenesis in Plasmodium falciparum [C] . C. Wagner, S.M. Scholz, A. Abreu, International Congress of Parasitology . 2006

机译：疟原虫在疟原虫发生过程中PFCCP多麦田粘附蛋白之间的分子相互作用
5. Development of a Viral Vaccine Vector Expressing a Plasmodium falciparum Erythrocyte Membrane Protein Domain to Target Severe Malaria [D] . Crager, Sara Eve 2011

机译：表达表达疟原虫红细胞膜蛋白结构膜蛋白域的病毒疫苗载体以靶向严重疟疾
6. Production crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6∊-FCR3 of the Plasmodium falciparum VAR2CSA protein. Corrigendum [O] . Anneleen Vuchelen, Els Pardon, Jan Steyaert, 2013

机译：针对恶性疟原虫VAR2CSA蛋白的达菲结合样（DBL）域DBL6∊-FCR3的两个纳米抗体的生产结晶和X射线衍射分析。更正
7. Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6∊-FCR3 of thePlasmodium falciparumVAR2CSA protein [O] . Anneleen Vuchelen, Els Pardon, Jan Steyaert, 2013

机译：两种纳米型抗肌腱粘合剂的制作，结晶和X射线衍射分析（DBL）结构域DBL6ε-FCR3的β-FBR6ε-FCR3

Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6-FCR3 of the Plasmodium falciparum VAR2CSA protein

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