Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana

Panneerselvam Saravanan; Kaljunen Heidi; Mueller-Dieckmann Jochen

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Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana

机译：拟南芥乙烯受体ETR1催化结构域的克隆，过表达，纯化和初步X射线分析

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Ethylene is a gaseous plant hormone which controls many aspects of plant growth and development. It is perceived by membrane-bound receptors with a similarity to bacterial two-component systems. The catalytic and ATP-binding domain of the histidine kinase domain of ETR1 from Arabidopsis thaliana has been cloned, overexpressed and crystallized. The protein was crystallized together with various nucleotides. Crystals obtained in the presence of ADP belonged to space group I222 or I2(1)2(1)2(1) with one molecule per asymmetric unit. They diffracted X-ray radiation to beyond 1.85 angstrom resolution.

机译：乙烯是一种气态植物激素，可控制植物生长和发育的许多方面。膜结合受体可以感知到它与细菌两组分系统的相似之处。已克隆，过表达和结晶了拟南芥ETR1组氨酸激酶结构域的催化和ATP结合结构域。该蛋白质与各种核苷酸一起结晶。在ADP存在下获得的晶体属于空间群I222或I2（1）2（1）2（1），每个不对称单元具有一个分子。他们将X射线辐射衍射到1.85埃以上的分辨率。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第11期|共3页
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Panneerselvam Saravanan; Kaljunen Heidi; Mueller-Dieckmann Jochen;
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1. Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana [J] . Panneerselvam Saravanan, Kaljunen Heidi, Mueller-Dieckmann Jochen Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第11期

机译：拟南芥乙烯受体ETR1催化结构域的克隆，过表达，纯化和初步X射线分析
2. GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis [J] . Li Kun, Yang Qingzhan, Wang Wei, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第11期

机译：拟南芥GPX3：克隆，表达，纯化，结晶和初步X射线分析
3. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AHP2, a signal transmitter protein from Arabidopsis thaliana [J] . Degtjarik Oksana, Dopitova Radka, Puehringer Sandra, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第2期

机译：拟南芥信号传递蛋白AHP2的克隆，表达，纯化，结晶和初步X射线衍射分析
4. PURIFICATION AND CHARACTERIZATION OF RECOMBINANT ETHYLENE RECEPTOR ETR1 FROM ARABIDOPSIS THALIANA [C] . Jan Voet-van-Vormizeele, Georg Groth International Congress of Photosynthesis . 2005

机译：拟南芥重组乙烯受体ETR1的纯化与表征
5. Over-expression of an ETR1/ERS1 ethylene receptor chimera in Arabidopsis thaliana. [D] . Mahoney, Justin J. 2005

机译：ETR1 / ERS1乙烯受体嵌合体在拟南芥中的过表达。
6. Cloning overexpression purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana [O] . Saravanan Panneerselvam, Heidi Kaljunen, Jochen Mueller-Dieckmann 2013

机译：拟南芥乙烯受体ETR1催化域的克隆过表达纯化和初步X射线分析
7. Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana. [O] . Panneerselvam, Saravanan, Kaljunen, Heidi, Mueller-Dieckmann, Jochen 2013

机译：拟南芥乙烯受体ETR1催化域的克隆，过表达，纯化和初步X射线分析。

Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana

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