...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Two-dimensional crystallization of intact F-ATP synthase isolated from bovine heart mitochondria
【24h】

Two-dimensional crystallization of intact F-ATP synthase isolated from bovine heart mitochondria

机译：从牛心脏线粒体分离的完整F-ATP合酶的二维结晶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mitochondrial F-ATP synthase produces the majority of ATP for cellular functions requiring free energy. The structural basis for proton motive force-driven rotational catalysis of ATP formation in the holoenzyme remains to be determined. Here, the purification and two-dimensional crystallization of bovine heart mitochondrial F-ATP synthase are reported. Two-dimensional crystals of up to 1 mm in size were grown by dialysis-mediated detergent removal from a mixture of decylmaltoside-solubilized 1,2-dimyristoyl-sn-glycero-3-phosphocholine and F-ATP synthase against a detergent-free buffer. A projection map calculated from an electron micrograph of a negatively stained two-dimensional crystal revealed unit-cell parameters of a = 185.0, b = 170.3 angstrom, gamma = 92.5 degrees.
机译：线粒体F-ATP合酶可产生大部分ATP，以实现需要自由能的细胞功能。质子动力驱动全酶中ATP形成的旋转催化的结构基础尚待确定。在此，报道了牛心脏线粒体F-ATP合酶的纯化和二维结晶。通过透析介导的去污剂从无麦芽糖苷溶解的1,2-二肉豆蔻酰基-sn-甘油-3-磷酸胆碱和F-ATP合酶的混合物中通过去污去污剂来生长最大尺寸为1 mm的二维晶体。从负染色二维晶体的电子显微照片计算得出的投影图揭示了a = 185.0，b = 170.3埃，γ= 92.5度的晶胞参数。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号