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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Purification, crystallization and preliminary X-ray crystallographic study of the tRNA pseudouridine synthase TruB from Streptococcus pneumoniae
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Purification, crystallization and preliminary X-ray crystallographic study of the tRNA pseudouridine synthase TruB from Streptococcus pneumoniae

机译：肺炎链球菌tRNA假尿苷合酶TruB的纯化，结晶和初步X射线晶体学研究

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摘要

The RNA pseudouridine synthase TruB catalyses the isomerization of uridine to pseudouridine (Psi) at residue 55 of elongator tRNAs. In order to better elucidate the functions of TruB in the formation of pseudouridine, the three-dimensional structure of full-length TruB was determined by X-ray crystallography. Here, the expression, purification, crystallization and preliminary crystallographic analysis of TruB from Streptococcus pneumoniae are reported. The crystal belonged to space group P2, with unit-cell parameters a = 37.65, b = 78.09, c = 56.33 angstrom, beta = 102.05 degrees, and diffracted to a resolution of 1.7 angstrom. The crystal is most likely to contain one molecule in the asymmetric unit, with a V-M value of 2.40 angstrom(3) Da(-1).
机译：RNA假尿苷合酶TruB在延伸子tRNA的55位残基处催化尿苷异构化为假尿苷（Psi）。为了更好地阐明TruB在假尿苷形成中的功能，通过X射线晶体学确定了全长TruB的三维结构。在此，报道了来自肺炎链球菌的TruB的表达，纯化，结晶和初步晶体学分析。晶体属于空间群P2，单位晶胞参数a = 37.65，b = 78.09，c = 56.33埃，β= 102.05度，并且衍射到1.7埃的分辨率。该晶体最有可能在不对称单元中包含一个分子，其V-M值为2.40埃（3）Da（-1）。

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