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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization studies of the keratin-like domain from Arabidopsis thaliana SEPALLATA 3
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Crystallization studies of the keratin-like domain from Arabidopsis thaliana SEPALLATA 3

机译:拟南芥SEPALLATA 3中角蛋白样结构域的结晶研究

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摘要

In higher plants, the MADS-box genes encode a large family of transcription factors (TFs) involved in key developmental processes, most notably plant reproduction, flowering and floral organ development. SEPALLATA 3 (SEP3) is a member of the MADS TF family and plays a role in the development of the floral organs through the formation of multiprotein complexes with other MADS-family TFs. SEP3 is divided into four domains: the M (MADS) domain, involved in DNA binding and dimerization, the I (intervening) domain, a short domain involved in dimerization, the K (keratin-like) domain important for multimeric MADS complex formation and the C (C-terminal) domain, a largely unstructured region putatively important for higher-order complex formation. The entire K domain along with a portion of the I and C domains of SEP3 was crystallized using high-throughput robotic screening followed by optimization. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 123.44, b = 143.07, c = 49.83 angstrom, and a complete data set was collected to 2.53 angstrom resolution.
机译:在高等植物中,MADS-box基因编码参与关键发育过程的最广泛的转录因子家族(TF),最显着的是植物繁殖,开花和花器官发育。 SEPALLATA 3(SEP3)是MADS TF家族的成员,通过与其他MADS家族TF形成多蛋白复合物,在花器官的发育中发挥作用。 SEP3分为四个域:参与DNA结合和二聚化的M(MADS)域,参与二聚化的短域I(介入)域,对于多聚MADS复合物形成重要的K(角蛋白样)域和C(C端)结构域,一个高度非结构化的区域,对高阶复合物的形成至关重要。 SEP3的整个K结构域以及I和C结构域的一部分使用高通量机器人筛选进行结晶,然后进行优化。晶体属于空间群P2(1)2(1)2,单位晶胞参数a = 123.44,b = 143.07,c = 49.83埃,并以2.53埃的分辨率收集了完整的数据集。

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