Crystallization and preliminary X-ray diffraction of the RNA demethylase ALKBH5

Zhou Bin; Han Zhifu

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Crystallization and preliminary X-ray diffraction of the RNA demethylase ALKBH5

机译：RNA脱甲基酶ALKBH5的结晶和初步X射线衍射

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N-6 -Methyladenosine (m6A) is a ubiquitous modification found in mammalian mRNA and long noncoding RNA. ALKBH5 is a member of the iron(II)-and 2-oxoglutarate-dependent AlkB oxygenase family and has been shown to catalyze the oxidative demethylation of N-6 -methyladenosine in RNA. The ALKBH5 protein was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.4 angstrom resolution using synchrotron radiation. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 57.456, b = 83.406, c = 92.909 angstrom, alpha = beta = gamma = 90.00 degrees and one molecule in the asymmetric unit.

机译：N-6-甲基腺苷（m6A）是在哺乳动物mRNA和长非编码RNA中普遍存在的修饰。 ALKBH5是依赖铁（II）和2-氧戊二酸的AlkB加氧酶家族的成员，并且已被证明可催化RNA中N-6-甲基腺苷的氧化脱甲基。使用悬滴蒸汽扩散法纯化ALKBH5蛋白并使其结晶。使用同步加速器辐射将晶体衍射至2.4埃分辨率。晶体属于空间群P2（1）2（1）2（1），其晶胞参数a = 57.456，b = 83.406，c = 92.909埃，α=β=伽马= 90.00度，并且其中一个分子位于不对称单位。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第11期|共4页
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Zhou Bin; Han Zhifu;
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1. Crystallization and preliminary X-ray diffraction of the RNA demethylase ALKBH5 [J] . Zhou Bin, Han Zhifu Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第11期

机译：RNA脱甲基酶ALKBH5的结晶和初步X射线衍射
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Crystallization and preliminary X-ray diffraction of the RNA demethylase ALKBH5

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