Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1

Wan Li; Williams Simon J.; Zhang Xiaoxiao; Ericsson Daniel J.; Koeck Markus; Dodds Peter N.; Ellis Jeffrey G.; Kobe Bostjan

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Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1

机译：亚麻细胞分裂素氧化酶LuCKX1.1的结晶和初步X射线衍射分析

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The plant hormones cytokinins play a central role in regulating cell division and developmental events. Cytokinin oxidase regulates the levels of these plant hormones by catalyzing their irreversible oxidation, which contributes to the regulation of various morpho-physiological processes controlled by cytokinins. In this study, the crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 are reported. Plate-like crystals of LuCKX1.1 were obtained using PEG 3350 as a precipitant and diffracted X-rays to 1.78 angstrom resolution. The protein crystals have the symmetry of space group C2 and are most likely to contain two molecules per asymmetric unit.

机译：植物激素细胞分裂素在调节细胞分裂和发育事件中起着核心作用。细胞分裂素氧化酶通过催化其不可逆的氧化作用来调节这些植物激素的水平，这有助于调节细胞分裂素控制的各种形态生理过程。在这项研究中，报道了亚麻细胞分裂素氧化酶LuCKX1.1的结晶和初步X射线衍射分析。使用PEG 3350作为沉淀剂，将X射线衍射至1.78埃分辨率，获得LuCKX1.1的板状晶体。蛋白质晶体具有C2空间群的对称性，每个不对称单元最有可能包含两个分子。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第10期|共3页
作者
Wan Li; Williams Simon J.; Zhang Xiaoxiao; Ericsson Daniel J.; Koeck Markus; Dodds Peter N.; Ellis Jeffrey G.; Kobe Bostjan;
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1. Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 [J] . Wan Li, Williams Simon J., Zhang Xiaoxiao, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第10期

机译：亚麻细胞分裂素氧化酶LuCKX1.1的结晶和初步X射线衍射分析
2. Crystallization and preliminary X-ray crystallographic analysis of pyridoxine 5'-phosphate oxidase complexed with flavin mononucleotide. [J] . Musayev FN, Di Salvo ML, Schirch V, Journal of Structural Biology . 1999,第1期

机译：吡ido醇5'-磷酸氧化酶与黄素单核苷酸复合的结晶和初步X射线晶体学分析。
3. Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus [J] . Feng-shan Gao, Lei Feng, Ping Jiang, BMC Veterinary Research . 2018,第1期

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4. X-ray Diffraction Analysis of the Effect of the NH4+: PO43- Molar Ratio on the Formation of Crystallized Struvite and Recovery Rate of Phosphorus [C] . Zongsheng Zhao, Xue Zou, Harry X. Zhang, The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议）论文集 . 2008

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5. Expression, purification, crystallization and preliminary X-ray analysis of PR-AMP cyclohydrolase, a zinc binding enzyme from Methanobacterium thermoautotrophicum. [D] . Boju, Lorena. 2004

机译：PR-AMP环水解酶的表达，纯化，结晶和初步X射线分析，PR-AMP环水解酶是一种来自嗜热自养甲烷杆菌的锌结合酶。
6. Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 [O] . Li Wan, Simon J. Williams, Xiaoxiao Zhang, 2013

机译：亚麻细胞分裂素氧化酶LuCKX1.1的结晶和初步X射线衍射分析
7. Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 [O] . Wan Li, Williams Simon J., Zhang Xiaoxiao, 2013

机译：亚麻细胞分裂素氧化酶LuCKX1.1的结晶和初步X射线衍射分析

Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1

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