Expression, purification, crystallization and preliminary X-ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles

Kikuchi Masaki; Iwabuchi Shinichiro; Kikkou Tatsuhiko; Noguchi Keiichi; Odaka Masafumi; Yohda Masafumi; Kawata Masaaki; Sato Chikara; Matsumoto Osamu

首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Expression, purification, crystallization and preliminary X-ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles

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Expression, purification, crystallization and preliminary X-ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles

机译：八面体颗粒对应的乙型肝炎病毒核心融合蛋白的表达，纯化，结晶和初步X射线晶体学研究

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Recombinant hepatitis B virus core proteins dimerize to form building blocks that are capable of self-assembly into a capsid. A core capsid protein dimer (CPD) linked to a green fluorescent protein variant, EGFP, at the C-terminus has been designed. The recombinant fusion CPD was expressed in Escherichia coli, assembled into virus-like particles (VLPs), purified and crystallized. The single crystal diffracted to 2.15 angstrom resolution and belonged to the cubic space group F432, with unit-cell parameters a = b = c = 219.7 angstrom. The fusion proteins assembled into icosahedral VLPs in aqueous solution, but were rearranged into octahedral symmetry through the crystal-packing process under the crystallization conditions.

机译：重组乙型肝炎病毒核心蛋白二聚形成可自我组装成衣壳的构件。已经设计了在C末端连接绿色荧光蛋白变体EGFP的核心衣壳蛋白二聚体（CPD）。重组融合CPD在大肠杆菌中表达，组装成病毒样颗粒（VLP），纯化并结晶。单晶衍射到2.15埃的分辨率，属于立方空间群F432，单位晶胞参数a = b = c = 219.7埃。融合蛋白在水溶液中组装成二十面体VLP，但在结晶条件下通过晶体堆积过程重新排列为八面体对称性。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第2期|共5页
作者
Kikuchi Masaki; Iwabuchi Shinichiro; Kikkou Tatsuhiko; Noguchi Keiichi; Odaka Masafumi; Yohda Masafumi; Kawata Masaaki; Sato Chikara; Matsumoto Osamu;
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1. Expression, purification, crystallization and preliminary X-ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles [J] . Kikuchi Masaki, Iwabuchi Shinichiro, Kikkou Tatsuhiko, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第2期

机译：八面体颗粒对应的乙型肝炎病毒核心融合蛋白的表达，纯化，结晶和初步X射线晶体学研究
2. Expression, purification, crystallization and preliminary X-ray crystallographic study of the nucleocapsid protein of Tomato spotted wilt virus [J] . Komoda Keisuke, Narita Masanori, Tanaka Isao, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第6期

机译：番茄斑萎病毒核衣壳蛋白的表达，纯化，结晶和X射线初步晶体学研究
3. Expression, crystallization and preliminary crystallographic study of mouse hepatitis virus (MHV) nucleocapsid protein C-terminal domain [J] . Xiaohang Tong Yanlin Ma Xuemei Li Acta Crystallographica Section F . 2010,第6期

机译：小鼠肝炎病毒（MHV）核衣壳蛋白C末端域的表达，结晶和初步晶体学研究
4. Overexpression and purification and particle self-assembly of a recombinant hepatitis B virus core derived anti-tumor protein from Escherichia coli [C] . Kong Jing, Deng Xiao-Zhao International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会) . 2009

机译：重组乙型肝炎病毒核心衍生的抗肿瘤蛋白的过表达，纯化和颗粒自组装
5. Chimeric orthohepadnavirus core particles for oral delivery of vaccines: Part I. Transformation of tobacco plants with a gene encoding a c-terminus truncated hepatitis B virus core protein. Part II. Construction of a woodchuck hepatitis virus core protein-based universal epitope carrier and test expression in Escherichia coli. [D] . Callaghan, Maximilian W. 2001

机译：用于口服递送疫苗的嵌合正丙肝病毒核心颗粒：第一部分。用编码c末端截短的乙型肝炎病毒核心蛋白的基因转化烟草植物。第二部分基于土拨鼠肝炎病毒核心蛋白的通用表位载体的构建和大肠杆菌中的表达测试。
6. Expression purification crystallization and preliminary X-ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles [O] . Masaki Kikuchi, Shinichiro Iwabuchi, Tatsuhiko Kikkou, 2013

机译：对应于八面体颗粒乙肝病毒核心融合蛋白的表达纯化结晶和初步X射线晶体学研究
7. Cloning, expression, purification, crystallization and preliminary crystallographic study of the protein module (BIV2-Helix) in the fusion core of bovine immunodeficiency-like virus (BIV) gp40 [O] . Zhao, Xiaodong, Li, Ming, Xu, Yanhui, 2005

机译：牛免疫缺陷样病毒（BIV）gp40融合核心蛋白模块（BIV2-Helix）的克隆，表达，纯化，结晶和初步晶体学研究

Expression, purification, crystallization and preliminary X-ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles

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