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Antibacterial photodynamic peptides for staphylococcal skin infection

机译：抗菌光动力学肽用于金葡萄球菌皮肤感染

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摘要

As the barrier between the human body and the outside world, the skin is vulnerable to pathogenic microorganisms, especially when suffering from skin injuries such as burns. Staphylococcus aureus remains the most common type of bacteria that infects humans, and the surging drug resistance poses a major threat to the treatment of these infections. Here we report the development of antibacterial photodynamic peptides (APPs) that are constructed based on the covalent conjugation of an antibacterial peptide and the photosensitizer chlorin e6 (Ce6). Peptide conjugation significantly increases the photo-stability of Ce6, while retaining its ROS generation capability under photo-irradiation. The APPs combine the antibacterial activity of the peptide and the photodynamic therapy of Ce6, and under the assistance of mild laser irradiation, can eradicate bacterial infection and inhibit the formation of bacterial biofilms ex vivo. One of the APPs, (GKRWWKWWRR)(2)KGGK(Ce6)G, AMP(2)-Ce6, with Ce6 conjugated with the dimeric peptide, showed exceptional antibacterial activity with an MIC90 value around 3.2 mu M without photo-irradiation and <0.1 mu M with short light treatment. Supported by a hydrogel matrix composed of gelatin and recombinant human collagen III protein (rhCol III) mimicking the extracellular matrix of skin cells, AMP(2)-Ce6 efficiently accelerated the healing rate of wounds and improved the quality of wound healing in mice infected with Staphylococcus aureus. Altogether, here we report the development of antibacterial photodynamic peptides, which together with a regenerative matrix material exhibit an added effect against staphylococcal skin infection. This composite material holds promise as a new type of wound dressing material for skin infection and wound healing.
机译：作为人体和外界之间的屏障，皮肤容易受到致病微生物的影响，特别是在患有烧伤等皮肤伤害时。金黄色葡萄球菌仍然是感染人类的​​最常见的细菌类型，并且汹涌的耐药性对这些感染的治疗产生了重大威胁。在这里，我们报告了基于抗菌肽和光敏剂氯e6（CE6）的共价缀合构建的抗菌光动力肽（APPS）的开发。肽缀合显着提高CE6的光稳定性，同时在光辐射下保留其ROS产生能力。该应用结合了肽的抗菌活性和CE6的光动力学治疗，并且在轻度激光照射的辅助下，可以消除细菌感染并抑制细菌生物膜前体内的形成。其中一个应用（GKRWKWWRR）（2）KGGK（CE6）G，AMP（2）-CE6，CE6与二聚体肽缀合的CE6，显示出卓越的抗菌活性，MIC90值约为3.2μm，没有照片辐照和< 0.1亩，短透光。由含明胶和重组人胶原III蛋白（rhcon III）组成的水凝胶基质，模仿皮肤细胞的细胞外基质，AMP（2）-CE6有效地加速伤口的愈合率，并改善了感染的小鼠中的伤口愈合质量金黄色葡萄球菌。在这里，我们报告了抗菌光动力肽的发展，其与再生基质材料一起表现出对葡萄球菌皮肤感染的额外效果。这种复合材料作为一种用于皮肤感染和伤口愈合的新型伤口敷料材料。

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  • 来源
    《Biomaterials Science》 |2020年第23期|共8页
  • 作者

    Lei Xiaoling; Qiu Lin; Lan Min; Du Xuancheng; Zhou Shuwen; Cui Pengfei; Zheng Ronghui; Jiang Pengju; Wang Jianhao; Xia Jiang;

  • 作者单位

    Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China;

    Shandong Univ Sch Phys Inst Adv Interdisciplinary Sci Jinan 250100 Peoples R China;

    Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China;

    Chinese Univ Hong Kong Dept Chem Shatin Hong Kong Peoples R China;

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  • 正文语种 eng
  • 中图分类 分子生物学;
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