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首页> 外文期刊>Biomaterials Science >SilkBridge (TM): a novel biomimetic and biocompatible silk-based nerve conduit
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SilkBridge (TM): a novel biomimetic and biocompatible silk-based nerve conduit

机译：Silkbridge（TM）：一种新型生物料和生物相容性丝绸神经管道

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Silk fibroin (Bombyx mori) was used to manufacture a nerve conduit (SilkBridge (TM)) characterized by a novel 3D architecture. The wall of the conduit consists of two electrospun layers (inner and outer) and one textile layer (middle), perfectly integrated at the structural and functional level. The manufacturing technology conferred high compression strength on the device, thus meeting clinical requirements for physiological and pathological compressive stresses. In vitro cell interaction studies were performed through direct contact assays with SilkBridge (TM) using the glial RT4-D6P2T cells, a schwannoma cell line, and a mouse motor neuron NSC-34 cell line. The results revealed that the material is capable of sustaining cell proliferation, that the glial RT4-D6P2T cells increased their density and organized themselves in a glial-like morphology, and that NSC-34 motor neurons exhibited a greater neuritic length with respect to the control substrate. In vivo pilot assays were performed on adult female Wistar rats. A 10 mm long gap in the median nerve was repaired with 12 mm SilkBridge (TM). At two weeks post-operation several cell types colonized the lumen. Cells and blood vessels were also visible between the different layers of the conduit wall. Moreover, the presence of regenerated myelinated fibers with a thin myelin sheath at the proximal level was observed. Taken together, all these results demonstrated that SilkBridge (TM) has an optimized balance of biomechanical and biological properties, being able to sustain a perfect cellular colonization of the conduit and the progressive growth of the regenerating nerve fibers.
机译：丝素素（BOMBYX MORI）用于制造神经管道（Silkbridge（TM）），其特征在于一种新的3D架构。导管的壁由两个电纺层（内部和外部）和一个纺织层（中间）组成，完美地集成在结构和功能水平。制造技术在该装置上赋予高压强度，从而满足生理和病理压缩应力的临床要求。通过使用GLIAL RT4-D6P2T细胞，氏葡萄瘤细胞系和小鼠电动机神经元NSC-34细胞直接接触测定通过直接接触测定进行体外细胞相互作用研究。结果表明，该材料能够维持细胞增殖，即GLIAL RT4-D6P2T细胞增加它们的密度并以胶质形态组织自己，并且NSC-34电机神经元相对于控件表现出更大的神经元长度基质。体内导频测定进行成年女性Wistar大鼠。中位神经中的10毫米长的间隙用12毫米丝网（TM）修复。在操作后两周后，几种细胞类型殖民化了内腔。在导管壁的不同层之间也可见细胞和血管。此外，观察到在近端水平下具有薄髓鞘鞘的再生髓鞘纤维的存在。总之，所有这些结果都表明Silkbridge（TM）具有优化的生物力学和生物特性平衡，能够维持导管的完美细胞定植和再生神经纤维的逐步生长。

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