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Stem-cell-derived ECM sheet-implant complexes for enhancing osseointegration

机译：干细胞衍生的ECM板植入复合物，用于增强骨整合

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摘要

Numerous treatment methods have been developed to modify the surface of dental implants to improve cell migration and proliferation, removal torque, and osseointegration. Recent studies have constructed cell sheet-implant complexes with enhanced osteogenic capabilities. However, these complexes have some limitations, such as requirements for complex preparation processes, cell vitality maintenance, strict preservation conditions, and the induction of immunogenicity. Extracellular matrix (ECM) sheets without cells may be a more desirable material. To date, the effect of ECM sheets on implant osseointegration has not been reported. In this study, we fabricated ECM sheet-implant complexes through the combination of rat bone marrow mesenchymal stem cell (BMSC)-derived ECM sheets with sandblasted, large-grit, acid-etched (SLA) implants. These complexes were characterized by light microscopy, scanning electron microscopy (SEM), and immunofluorescence (IF) assays. The adhesion, proliferation, and osteogenic differentiation of BMSCs cultured on ECM sheets were detected in vitro. Then, the ECM sheet-implant complexes were transplanted into the metaphysis of the tibias of rats to evaluate the implant osseointegration in vivo. The results showed that ECM sheets were successfully constructed and showed significantly improved adhesion and proliferation. BMSCs cultured on ECM sheets upregulated the expression levels of the osteogenic-related genes alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (Runx2) compared to controls. In vivo, ECM sheet-implant complexes demonstrated superior new bone formation. Our findings proved that the BMSC-derived ECM sheets promoted osseointegration in vitro and in vivo. The current study indicated that the ECM sheet could be an ideal tissue engineering material, and ECM sheet-implant complexes could provide a strategy with low immunogenicity and easy storage and transportation. This research provides a novel strategy for the development of implant surface modification approaches.
机译：已经开发了许多治疗方法以改变牙科植入物的表面，以改善细胞迁移和增殖，去除扭矩和骨整合。最近的研究已经构建了具有增强的成骨能力的细胞片植入物复合物。然而，这些配合物具有一些局限性，例如复杂的制备过程的要求，细胞活力维持，严格的保存条件以及免疫原性的诱导。没有细胞的细胞外基质（ECM）片材可以是更理想的材料。迄今为止，尚未报告ECM纸张对植入骨整合的影响。在这项研究中，我们通过大鼠骨髓间充质干细胞（BMSC）的ECM板与喷砂，大型酸蚀刻（SLA）植入物的组合制造ECM板植入复合物。通过光学显微镜，扫描电子显微镜（SEM）和免疫荧光（IF）测定，这些复合物的特征在于。在体外检测在ECM板上培养的BMSCs的粘附性，增殖和成骨分化。然后，将ECM板植入复合物移植到大鼠胫骨的胫骨膜中，以评估体内植入骨整极。结果表明，ECM板材成功构建并显示出显着改善的粘附性和增殖。与对照相比，在ECM纸上培养的BMSCs上调了骨质发生相关基因碱性磷酸酶（ALP），骨形态发生蛋白2（BMP2）和RUNT相关转录因子2（RUNX2）的表达水平。在体内，ECM板植入复合物展示了优异的新骨形成。我们的研究结果证明，BMSC衍生的ECM纸张在体外和体内促进了骨整合。目前的研究表明，ECM板可以是理想的组织工程材料，ECM板植入复合物可以提供低免疫原性和易于储存和运输的策略。本研究提供了一种新颖的植入面修饰方法的开发策略。

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  • 来源
    《Biomaterials Science》 |2020年第23期|共10页
  • 作者

    Feng Yuting; Jiang Zhiwei; Zhang Yanmin; Miao Xiaoyan; Yu Qiong; Xie Zhijian; Yang Guoli;

  • 作者单位

    Zhejiang Univ Affiliated Hosp Stomatol Med Coll Dept Implantol 395 Yanan Rd Hangzhou 310006 Zhejiang Peoples R China;

    Zhejiang Univ Affiliated Hosp Stomatol Med Coll Dept Implantol 395 Yanan Rd Hangzhou 310006 Zhejiang Peoples R China;

    Zhejiang Univ Affiliated Hosp Stomatol Med Coll Dept Implantol 395 Yanan Rd Hangzhou 310006 Zhejiang Peoples R China;

    Key Lab Oral Biomed Res Zhejiang Prov Hangzhou Peoples R China;

    Zhejiang Univ Affiliated Hosp Stomatol Med Coll Dept Implantol 395 Yanan Rd Hangzhou 310006 Zhejiang Peoples R China;

    Key Lab Oral Biomed Res Zhejiang Prov Hangzhou Peoples R China;

    Zhejiang Univ Affiliated Hosp Stomatol Med Coll Dept Implantol 395 Yanan Rd Hangzhou 310006 Zhejiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子生物学;
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